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We narrowed to 9,088 results for: ef1;

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Showing: 3201 - 3250 of 9088 results

  2. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Antonella Fidanza, Lesley Forrester
    Article
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211495-plasmid-map-sequence-id-454077

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  3. TFORF0418

    Plasmid
    #142259
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CDC5L (CDC5L Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142259-plasmid-map-sequence-id-395751

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  4. TFORF2693

    Plasmid
    #142359
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD4 (TEAD4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142359-plasmid-map-sequence-id-397176

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  7. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  8. PB-iRFP-STOP-ReNL

    Plasmid
    #113965
    Purpose
    Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Inserts
    iRFP670
    ReNL
    Use
    Piggybac transposon
    Expression
    Mammalian
    Promoter
    CMV - SV40-PolyA - SV40-PolyA and EF1A
    Available Since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    113965-plasmid-map-sequence-id-223037

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  11. TFORF3498

    Plasmid
    #144974
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    APEX1 (APEX1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144974-plasmid-map-sequence-id-404544

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  15. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  22. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  25. makeTCR_Lentiviral_Backbone

    Plasmid
    #233943
    Purpose
    backbone for makeTCR assembly
    Depositor
    Michael Platten
    Type
    Empty backbone
    Use
    Lentiviral
    Promoter
    EF1a (long)
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    233943-plasmid-map-sequence-id-469998

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  26. pEBB-SBP-HA

    Plasmid
    #36100
    Depositor
    Ezra Burstein
    Type
    Empty backbone
    Tags
    HA and SBP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    36100-plasmid-map-sequence-id-44719

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  27. pLX_TRC311

    Plasmid
    #113668
    Purpose
    Gateway destination vector for lentiviral expression
    Depositor
    John Doench
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113668-plasmid-map-sequence-id-220922

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  28. BII-ChPtW-tdT

    Plasmid
    #133401
    Purpose
    Piggybac vector for constitutive expression of tdTomato
    Depositor
    Blair Madison
    Insert
    tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133401-plasmid-map-sequence-id-268578

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  29. BII-ChPtW-iRFP702

    Plasmid
    #133398
    Purpose
    Piggybac vector for constitutive expression of iRFP702
    Depositor
    Blair Madison
    Insert
    IRFP-702
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133398-plasmid-map-sequence-id-267650

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  30. BII-ChPtW-iRFP670

    Plasmid
    #133397
    Purpose
    Piggybac vector for constitutive expression of iRFP670-NLS
    Depositor
    Blair Madison
    Insert
    iRFP-670-NLS
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133397-plasmid-map-sequence-id-269430

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  31. BII-ChPtW-smURFP

    Plasmid
    #133399
    Purpose
    Piggybac vector for constitutive expression of smURFP-NLS
    Depositor
    Blair Madison
    Insert
    smURFP-NLS
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133399-plasmid-map-sequence-id-268584

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  32. lentiCRISPRv2_ZsG gRNA_4

    Plasmid
    #192688
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    192688-plasmid-map-sequence-id-379587

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  33. lentiCRISPRv2_ZsG gRNA_1

    Plasmid
    #192685
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192685-plasmid-map-sequence-id-379591

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  34. lentiCRISPRv2_ZsG gRNA_2

    Plasmid
    #192686
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192686-plasmid-map-sequence-id-379590

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  35. lentiCRISPRv2_ZsG gRNA_3

    Plasmid
    #192687
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192687-plasmid-map-sequence-id-379588

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  36. pDY0973-RADARS iCaspase

    Plasmid
    #182539
    Purpose
    Express RADARS iCaspase that conditionally turn on iCaspase in the presence of target RNA
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Insert
    iCaspase9
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182539-plasmid-map-sequence-id-356646

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  37. pJY4092

    Plasmid
    #113334
    Purpose
    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter
    Depositor
    Suzanne Sandmeyer
    Insert
    codon-optimized Hermes transposase
    Expression
    Yeast
    Available Since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113334-plasmid-map-sequence-id-218285

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  38. piggyBAC-U6-GFP-WPRE

    Plasmid
    #200030
    Purpose
    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Jay Shendure
    Type
    Empty backbone
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    U6, EF1a
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200030-plasmid-map-sequence-id-393204

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  39. BII-Sh-BnTW

    Plasmid
    #133365
    Purpose
    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    Flag-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133365-plasmid-map-sequence-id-268571

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  40. pCMX-Gal4-TEAD1-D278K

    Plasmid
    #38106
    Depositor
    Kunliang Guan
    Insert
    TEAD1 (TEAD1 Human)
    Tags
    Gal4-DBD
    Expression
    Mammalian
    Mutation
    D278K
    Available Since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  41. Lenti NbALFA-mNeonGreen puro

    Plasmid
    #193973
    Purpose
    lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, puromycin resistance
    Depositor
    Raphael Gaudin
    Insert
    NbALFA-mNeonGreen
    Use
    Lentiviral
    Tags
    mNeonGreen
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    193973-plasmid-map-sequence-id-384356

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  42. Lenti NbALFA-mNeonGreen hygro

    Plasmid
    #193974
    Purpose
    lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, hygromycin resistance
    Depositor
    Raphael Gaudin
    Insert
    NbALFA-mNeonGreen
    Use
    Lentiviral
    Tags
    mNeonGreen
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    193974-plasmid-map-sequence-id-384357

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  43. pLV Rnf121-V5

    Plasmid
    #175130
    Purpose
    Lentiviral expression of mouse Rnf121-V5
    Depositor
    Larry Gerace
    Insert
    Rnf121 (Rnf121 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175130-plasmid-map-sequence-id-352014

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  44. Lenti NbALFA-miRFP680 hygro

    Plasmid
    #193969
    Purpose
    lentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, hygromycin resistance
    Depositor
    Raphael Gaudin
    Insert
    NbALFA-miRFP680
    Use
    Lentiviral
    Tags
    miRFP680
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    193969-plasmid-map-sequence-id-384355

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  45. pLenti-shOGDH #2

    Plasmid
    #242705
    Purpose
    shRNA knockdown human OGDH gene
    Depositor
    Xingguo Zhu
    Insert
    OGDH (OGDH Human)
    Use
    Lentiviral
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242705-plasmid-map-sequence-id-487618

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  46. pLenti-shOGDH #1

    Plasmid
    #242704
    Purpose
    shRNA knockdown human OGDH gene
    Depositor
    Xingguo Zhu
    Insert
    OGDH (OGDH Human)
    Use
    Lentiviral
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242704-plasmid-map-sequence-id-487614

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  47. Lenti NbALFA-miRFP680 puro

    Plasmid
    #193972
    Purpose
    lentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, puromycin resistance
    Depositor
    Raphael Gaudin
    Insert
    NbALFA-miRFP680
    Use
    Lentiviral
    Tags
    miRFP680
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    193972-plasmid-map-sequence-id-384354

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  48. pLV V5-Mospd1

    Plasmid
    #175132
    Purpose
    Lentiviral expression of V5-tagged mouse Mospd1
    Depositor
    Larry Gerace
    Insert
    Mospd1 (Mospd1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175132-plasmid-map-sequence-id-352019

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  50. pSBbi-pur H-2Kb

    Plasmid
    #111623
    Purpose
    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele
    Depositor
    Jon Yewdell
    Insert
    H2Kb
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111623-plasmid-map-sequence-id-213220

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Showing: 3201 - 3250 of 9088 results