We narrowed to 2,422 results for: NIG

1. pcs2-N-HRI(1-138)-GFP-IRES-mCherry

   Plasmid

   #226100

   Purpose

   HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains only N-HRI (aa 1-138)

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-3xFlag-SPNS1

   Plasmid

   #198568

   Purpose

   SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1 with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC1_oROS-HT_LF(C199S)

   Plasmid

   #216412

   Purpose

   Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT_LF(C199S)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1-lifeact-oROS-HT

   Plasmid

   #216420

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   lifeact-oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLIK mCh-V5-NES-EGFP-EGFR NLS zeo

   Plasmid

   #192337

   Purpose

   Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLIK mCh-V5-NES-EGFP-Erbb2 NLS zeo

   Plasmid

   #192339

   Purpose

   Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN_TT mCh-V5-NES-EGFP-EGFR NLS

   Plasmid

   #192315

   Purpose

   Gateway entry vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_TT mCh-V5-NES-EGFP-Erbb2 NLS

   Plasmid

   #192317

   Purpose

   Gateway entry vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe NGF-ERBB2-2xFKBP-HA hygro

   Plasmid

   #192292

   Purpose

   Retroviral expression vector for NGF-ERBB2 -2xFKBP-HA construct

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   NGF-ERBB2-2xFKBP-HA (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mWasabi-ADAP1

    Plasmid

    #163906

    Purpose

    Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Use

    Tags

    mWasabi fluorescent tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA BRCA2 S3070F

    Plasmid

    #139325

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 S3070F using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 S3070F using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sgRNA BRCA1 S1363L

    Plasmid

    #139329

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 S1363L using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 S1363L using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgRNA BRCA1 E1754K

    Plasmid

    #139330

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 E1754K using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 E1754K using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sgRNA FANCD2 Q223Stop

    Plasmid

    #139332

    Purpose

    Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert FANCD2 Q223Stop using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-VRER-P2A-Puro

    Plasmid

    #110849

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-HF1-P2A-Puro

    Plasmid

    #110850

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-VQR-PGK-Puro

    Plasmid

    #110855

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-VRER-PGK-Puro

    Plasmid

    #110856

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCHKU27.2-2.2

    Plasmid

    #110662

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLuc-OptoJNKi

    Plasmid

    #89744

    Purpose

    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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