We narrowed to 12,364 results for: NUC

1. M-SPn-VP64

   Plasmid

   #48674

   Purpose

   Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Expression

   Mutation

   nuclease-null

   Promoter

   CMV

   Available since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pYZ714

   Plasmid

   #132955

   Purpose

   crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

   Insert

   dFnCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mutation

   Promoter

   adh1

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SARS-CoV-2_N_deltaNTD

   Plasmid

   #157869

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   deltaNTD

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKF257

   Plasmid

   #52533

   Purpose

   expresses I-SceI nuclease in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Tags

   HA and NLS

   Expression

   Insect

   Mutation

   Promoter

   tubulin

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. SARS-CoV-2_N_deltaNIDR

   Plasmid

   #157868

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDR

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   deltaNIDR

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcGFP-Jpx9C104T

   Plasmid

   #120381

   Purpose

   Reporter for RNA nuclear localization

   Depositor

   Igor Ulitsky

   Article

   Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

   Insert

   JPX (JPX Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   C321T

   Promoter

   CMV

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7-RelA(K221R)

   Plasmid

   #23248

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   NFkB RelA (RELA Human)
   

   Use

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   K221R

   Promoter

   Available since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pCMV HDM2(NES-)

   Plasmid

   #12087

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   HDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Nuclear-export sequence mutant (NES-): L205A, I20…

   Promoter

   Available since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

9. T7-RelA(K218R)

   Plasmid

   #23247

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   NFkB RelA(K218R) (RELA Human)
   

   Use

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   K218R

   Promoter

   Available since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pAL1

    Plasmid

    #197285

    Purpose

    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCLR2068

    Plasmid

    #28062

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains H537R point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SARS-CoV-2_N_deltaCIDR

    Plasmid

    #157872

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDR

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    deltaCIDR

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. NET39 pEGFP-C1 (1045)

    Plasmid

    #62024

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET39 (PPAPDC3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

15. LDB204

    Plasmid

    #185426

    Purpose

    Bacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusion

    Depositor

    Laura Davis, Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 FXFG-repeats domain expressed

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAcGFP-Jpx9

    Plasmid

    #120379

    Purpose

    Reporter for RNA nuclear localization

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    JPX (JPX Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS315e_pGal-dCas9-SH3

    Plasmid

    #79613

    Purpose

    Expresses dCas9-SH3 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    SH3 domain

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NET39 mRFP-C1 (1219)

    Plasmid

    #62042

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET39 (PPAPDC3 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAcGFP-Jpx9C104A

    Plasmid

    #120380

    Purpose

    Reporter for RNA nuclear localization

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    JPX (JPX Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C321A

    Promoter

    CMV

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPTXLUC

    Plasmid

    #68504

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    OPTX

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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