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We narrowed to 63,651 results for: cloning

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Showing: 1301 - 1320 of 63651 results

  1. PB-U6-gRNA-Ef1a-CRISPRi-blast

    Plasmid
    #262893
    Purpose
    piggyBac vector to transduce both U6-driven gRNA and Ef1a-driven CRISPRi with blasticidin resistance
    Depositor
    Ronald Hart
    Inserts
    U6-gRNA Cloning Site (BsmB1 GoldenGate)
    KRAB-dCas9-MeCP2
    Use
    CRISPR; Piggybac
    Tags
    blasticidin S deaminase
    Expression
    Mammalian
    Promoter
    Ef1a and U6
    Available since
    Oct. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    262893-plasmid-map-sequence-id-524313

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  2. pcU6_3 MS2 sgRNA

    Plasmid
    #92394
    Purpose
    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    chick U6.3
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92394-plasmid-map-sequence-id-191866

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  14. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway Cloning
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122843-plasmid-map-sequence-id-244374

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  15. pVITRO1-Trastuzumab-IgG4/κ

    Plasmid
    #61887
    Purpose
    Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61887-plasmid-map-sequence-id-521184

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  20. ZsGreen1-N1

    Plasmid
    #54702
    Purpose
    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    ZsGreen1
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54702-plasmid-map-sequence-id-266653

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Showing: 1301 - 1320 of 63651 results