We narrowed to 1,387 results for: pBAD

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pBN076

Plasmid

#167957

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN077

Plasmid

#167958

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN078

Plasmid

#167959

Purpose

express E. coli E69 (O9a:K30) Wzc with Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-3

Plasmid

#164867

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-3

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-2

Plasmid

#164866

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-2

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDae079 (eMutaT7[transition])

Plasmid

#187622

Purpose

Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Inserts

TadA-8e-T7RNAP
PmCDA1-T7RNAP

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDae069 (eMutaT7[PmCDA1] with optimized RBS)

Plasmid

#187621

Purpose

Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

PmCDA1-T7RNAP

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSiMPlk_N + pSiMPlk_C

Plasmid

#134310

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

kanR_gp41-1 N-intein + kanR_gp41-1 C-intein

Expression

Bacterial

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBLADE_ONLY_C

Plasmid

#168050

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Insert

Blue light-inducible AraC dimers in Escherichia coli

Expression

Bacterial

Promoter

J23101**

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pPbuCas13b

Plasmid

#184565

Purpose

Inducible expression of PbuCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Prevotella buccae

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSiMPlc_N + pSiMPlc_C

Plasmid

#134312

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

cmR_gp41-1 N-intein + cmR_gp41-1 C-intein

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSiMPla_N + pSiMPla_C

Plasmid

#134314

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

ampR_gp41-1 N-intein + ampR_gp41-1 C-intein

Expression

Bacterial

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSiMPlh_N + pSiMPlh_C

Plasmid

#134316

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

hygR_gp41-1 N-intein + hygR_gp41-1 C-intein

Expression

Bacterial

Mutation

P254Q in hygR_gp41-1 N-intein

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcF-1

Plasmid

#131023

Purpose

Single spacer array with spacer #1 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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CBla_2.0_EmrE_TMD4_3P

Plasmid

#207848

Purpose

BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

C-BLa 2.0 fusion protein

Tags

Flag (DYKDHDG)

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBN415

Plasmid

#217920

Purpose

express E. coli E69 (O9a:K30) Wzc with N711Y mutation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:

Insert

E. coli E69 (O9a:K30) Wzc with N711Y mutation

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

N711Y

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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CBLa_1.2_GpA_wt

Plasmid

#207843

Purpose

BLaTM-System GpA wt positive control in CBLa 1.2 vector

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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CBLa_1.2_GpA_G83I

Plasmid

#207845

Purpose

BLaTM-System GpA G83I negative control in CBLa 1.2 vector

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBN086

Plasmid

#173448

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y715E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y715E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y715E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN087

Plasmid

#173449

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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