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We narrowed to 18,567 results for: puro

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Showing: 1301 - 1320 of 18567 results

  3. m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211342
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211342-plasmid-map-sequence-id-415648

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  4. m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211344
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211344-plasmid-map-sequence-id-415651

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  5. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  6. qTAG-N-Puro-mStayGold-ACTB

    Plasmid
    #227320
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227320-plasmid-map-sequence-id-452091

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  7. XLone-Puro SPI1 P2A mCherry

    Plasmid
    #140029
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140029-plasmid-map-sequence-id-275844

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  15. pMSCVpuro-Mito-DsRed1

    Plasmid
    #87379
    Purpose
    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).
    Depositor
    Björn Stork
    Publication
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    TXN2 mitochondrial transit peptide (TXN2 Human)
    Use
    Retroviral
    Tags
    DsRed1
    Expression
    Mammalian
    Mutation
    First 60 aa only
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87379-plasmid-map-sequence-id-212256

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  16. pLVXPuro-mGFP-LIC1

    Plasmid
    #66600
    Purpose
    Expresses Dynein-1 LIC1 as a mGFP fusion
    Depositor
    David Stephens
    Insert
    LIC1 (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    mGFP
    Expression
    Mammalian
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

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Showing: 1301 - 1320 of 18567 results