165,385 results

1. pUCM-CLYBL-NGN2-BRN3A

   Plasmid

   #165597

   Purpose

   Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

   Depositor

   Carsten Bönnemann

   Article

   Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   BRN3A (POU4F1 Human)
   EGFP
   Puromycin N-acetyl transferase
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Expression

   Mammalian

   Promoter

   CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

   Available Since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

   Plasmid

   #213117

   Purpose

   Expression of a faster-running MinE mutant in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE(L4E)-EGFP (minE Escherichia coli)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   L4E

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

   Plasmid

   #213116

   Purpose

   Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   FRB-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mFGF2

   Plasmid

   #22084

   Depositor

   Gail Martin

   Insert

   FGF2 (Fgf2 Mouse)
   

   Use

   Bacterial

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pMK290 (mAID-mClover-Hygro)

   Plasmid

   #72828

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Hygro
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGFP-LacI

   Plasmid

   #179510

   Purpose

   Expresses EGFP-LacI in mammalian cells

   Depositor

   Yasushi Hiraoka

   Article

   Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

   Insert

   LacI
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223 SARS-CoV-2 NSP6

   Plasmid

   #141260

   Purpose

   Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. PIP2-GFP(ST) sensor

   Plasmid

   #170356

   Purpose

   GFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

   Depositor

   Kees Jalink

   Article

   van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

   Insert

   GFP(ST)-PH (PLCD1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSW002-PpsbA_mTurquoise2

   Plasmid

   #108237

   Purpose

   Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   PsbA promoter - mTurquoise2
   

   Expression

   Bacterial

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1_puro_shNRF2-1

    Plasmid

    #230086

    Purpose

    shRNA silencing human NRF2 gene

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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