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pWZL-Blast FAPwt

Plasmid

#210643

Purpose

Expresses the FAP (Fibroblast Activation Protein) protein.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

none

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Venus-Akaluc (neo)

Plasmid

#124701

Purpose

Aka-Luciferase reporter

Depositor

Roland Friedel

Article

Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

Insert

Venus-Akaluciferase

Use

Lentiviral

Promoter

hPGK

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTO135_pENO1-CaCas9-tCyc1

Plasmid

#171103

Purpose

Expresses Cas9 in Candida auris

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

CaCas9

Expression

Yeast

Promoter

ENO1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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M13cp-dg3 hp

Plasmid

#218095

Purpose

Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-BirA*-dCas9-eGFP

Plasmid

#84779

Purpose

Expresses BirA*-dCas9-eGFP in mammalian cells.

Depositor

Heinrich Leonhardt

Article

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Insert

BirA*-dCas9-eGFP

Use

CRISPR

Tags

cMyc

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

July 29, 2017

Availability

Academic Institutions and Nonprofits only

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hSERT pcDNA3

Plasmid

#15483

Depositor

Randy Blakely

Article

Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

Insert

hSERT (SLC6A4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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pTP1122

Plasmid

#104159

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

Plasmid

#216156

Purpose

Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with C-terminal FLAG tag

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Golgi

Plasmid

#36205

Purpose

In vivo visualization of golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Golgi targeting sequence (B4GALT1 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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