We narrowed to 5,102 results for: codon optimized

1. pET11d-FHis-DND1-Y102A

   Plasmid

   #70082

   Purpose

   Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (DND1 Human)
   

   Tags

   6xHIS and FLAG

   Expression

   Bacterial

   Mutation

   Y102A. Codon optimized for bacterial expression

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. LeGO-iBSD-5LO-d4

   Plasmid

   #182743

   Purpose

   Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).

   Depositor

   Dieter Steinhilber

   Article

   Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

   Insert

   5-Lipoxygenase isoform delta 4 (ALOX5 Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   codon optimized for expression in homo sapiens

   Promoter

   SFFV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LeGO-iBSD-5LO-p12

   Plasmid

   #182744

   Purpose

   Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform p12) in mamallian cells with blasticidin S resistance (bsd).

   Depositor

   Dieter Steinhilber

   Article

   Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

   Insert

   5-Lipoxygenase isoform p12 (ALOX5 Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   codon optimized for expression in homo sapiens

   Promoter

   SFFV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LeGO-iBSD-5LO-E376Q

   Plasmid

   #182742

   Purpose

   Lentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).

   Depositor

   Dieter Steinhilber

   Article

   Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

   Insert

   5-Lipoxygenase E376Q (ALOX5 Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   enzymatically inactive 5-LO E376Q mutant, codon o…

   Promoter

   SFFV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LeGO-iBSD-5LO-d13

   Plasmid

   #182745

   Purpose

   Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).

   Depositor

   Dieter Steinhilber

   Article

   Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

   Insert

   5-Lipoxygenase isoform delta 13 (ALOX5 Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   codon optimized for expression in homo sapiens

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH848-gst-disec

   Plasmid

   #89589

   Purpose

   Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase codon-dis-optimised for bacterial expression
   

   Tags

   8xhistidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD10AH840AhCas9 (GB1041)

   Plasmid

   #68207

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNHT7::UMSBP::sNluc2_ORF2

   Plasmid

   #186766

   Purpose

   An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNHT7::RPL15::sNluc2_STOP

   Plasmid

   #186767

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Mutation

   Premature stop codon at residue 16

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GalL

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. p414-TEF1p-Cas9-CYC1t

    Plasmid

    #43802

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFC330

    Plasmid

    #87842

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC1749

    Plasmid

    #127983

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFC331

    Plasmid

    #87843

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    argB
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC1750

    Plasmid

    #127984

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAC1748

    Plasmid

    #127982

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDL0_029

    Plasmid

    #210761

    Purpose

    SC_Oryza sativa codon-optimized LHGR-N(Ligand binding domain and rat glucocorticoid receptor (rcoLHGR-N)

    Depositor

    Dmitri Nusinow

    Article

    Lee et al bioRxiv 2025.06.20.660755

    Insert

    rice codn optimized LHGRN
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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