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pJRTCLCrVA009

Plasmid

#22842

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA containing CHLI fragment

Use

Cotton vigs vector

Expression

Bacterial

Mutation

Cotton Leaf Crumple Virus Coat Protein (AR1 gene)…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pTet02

Plasmid

#78284

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-5

Plasmid

#109011

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #5 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_tnnt2a_cmlc2-nmKate

Plasmid

#238309

Purpose

Drives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mVenus-rRim1[20-227]-mVenus(L68V)

Plasmid

#246280

Purpose

Rab10 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

Venus-rRim1[20-227]-mVenus(L68V) (Rims1 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

Plasmid

#225438

Purpose

Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-FRT-OCS-BeYDVRepRepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)

Plasmid

#225437

Purpose

Transcriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-att-OCS-BeYDVRep/RepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

Plasmid

#225430

Purpose

OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-FRT-OCSterm-FRT-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)

Plasmid

#225431

Purpose

OCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-att-OCSterm-att-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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MIGR1_FLAG_TAL1-long_dt tomato

Plasmid

#210639

Purpose

Overexpression of TAL1-long

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-long (TAL1 Human)

Use

Retroviral

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV eGFP

Plasmid

#206272

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

eGFP

Use

Multimate/gateway entr 4

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV mTagBFP

Plasmid

#206279

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mTagBFP

Use

Multimate/gateway entr 4

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg6-sh(t10)RNAres_S

Plasmid

#147460

Purpose

Mammalian Expression of HsSmg6-sh(10)RNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg6-sh(10)RNAres (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7-siRNAres-shRNAres_S

Plasmid

#147463

Purpose

Mammalian Expression of HsSmg7-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres-shRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-G100E-siRNAres-V5-SBP-MBP_S

Plasmid

#147472

Purpose

Mammalian Expression of HsSmg7-G100E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-G100E-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-K66ER163E-siRNAres-V5-SBP-MBP_S

Plasmid

#147473

Purpose

Mammalian Expression of HsSmg7-K66ER163E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-K66ER163E-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-siRNAres-V5-SBP-MBP_S

Plasmid

#147474

Purpose

Mammalian Expression of HsSmg7-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1_115-1129-S1107A-siRNAres_S

Plasmid

#147477

Purpose

Mammalian Expression of HsUpf1_115-1129-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1_115-1129-S1107A-siRNAres (UPF1 Human)

Expression

Mammalian

Mutation

4 non silent mutations E167G, K282R, A442G and T1…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1-S1107A-siRNAres_S

Plasmid

#147478

Purpose

Mammalian Expression of HsUpf1-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1-S1107A-siRNAres (UPF1 Human)

Expression

Mammalian

Mutation

5 non silent mutations A69S, E167G, K282R, A442G…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-TBG-Cre-sgArid1a

Plasmid

#192165

Purpose

AAV-TBG-Cre-sgArid1a

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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