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Showing: 1321 - 1340 of 15241 results

  2. lentiCRISPR - EGFP sgRNA 3

    Plasmid
    #51762
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51762-plasmid-map-sequence-id-79380

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  3. pSmart-Nm-sgRNA-BbsI

    Plasmid
    #49157
    Purpose
    Plasmid for cloning spacer into sgRNA for NmCas9
    Depositor
    James Thomson
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    49157-plasmid-map-sequence-id-72446

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  4. Plas-gRNA-Z3EV

    Plasmid
    #157659
    Purpose
    GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.
    Depositor
    Frank Albert
    Article
    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1
    Insert
    GFP-gRNA NatMX Z3EV
    Use
    Insert storage (replicative in e. coli)
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    157659-plasmid-map-sequence-id-309102

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  5. pLenti-gRNA-puro

    Plasmid
    #180426
    Purpose
    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    gRNAscr
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    180426-plasmid-map-sequence-id-354805

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  11. pCMV-BFP-U6-sgRNA

    Plasmid
    #196725
    Purpose
    Sortable sgRNA expression, Chen tracrRNA PMID: 24360272
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196725-plasmid-map-sequence-id-388067

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Showing: 1321 - 1340 of 15241 results