We narrowed to 15,965 results for: grna

1. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ERBB2 gRNA (BRDN0001148673)

   Plasmid

   #76377

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ERBB2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AXL gRNA (BRDN0001146797)

   Plasmid

   #76700

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human AXL

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   AXL (AXL Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PU6::klp-12_sgRNA

   Plasmid

   #46170

   Purpose

   klp-12 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   klp-12 targeting sgRNA (klp-12 Synthetic)
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available Since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-HypaCas9-RHOA_sgRNA

   Plasmid

   #183878

   Purpose

   pX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA sgRNA spacer (RHOA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

   Plasmid

   #85453

   Purpose

   Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-sgRNA-Backbone-UP-TdTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   deletion of AA123-365 in tdTomato (please see dep…

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AXL gRNA (BRDN0001147657)

   Plasmid

   #76701

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human AXL

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   AXL (AXL Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KIF20A D5.2 gRNA

   Plasmid

   #90718

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KIF20A

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   KIF20A (Guide Designation D5.2)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MAP2K2 gRNA (BRDN0001147718)

   Plasmid

   #77601

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP2K2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP2K2 (MAP2K2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AXL gRNA (BRDN0001146919)

    Plasmid

    #76702

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AXL

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    AXL (AXL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX462-hPRKAA2-gRNA_B

    Plasmid

    #74377

    Purpose

    gRNA_B to knockout human AMPK alpha 2 using Cas9n

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MAP4K4 gRNA (BRDN0001162353)

    Plasmid

    #76266

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP4K4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP4K4 (MAP4K4 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MAP4K4 gRNA (BRDN0001148610)

    Plasmid

    #76263

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP4K4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP4K4 (MAP4K4 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MAP4K4 gRNA (BRDN0001162594)

    Plasmid

    #76264

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP4K4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP4K4 (MAP4K4 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DKC1 F1.3 gRNA

    Plasmid

    #90652

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DKC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DKC1 (Guide Designation F1.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAP2K2 gRNA (BRDN0001147931)

    Plasmid

    #77603

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP2K2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MAPK3 gRNA (BRDN0001147876)

    Plasmid

    #75967

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MLKL gRNA (BRDN0001149268)

    Plasmid

    #77541

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MLKL

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MLKL (MLKL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiCRISPR - EGFP sgRNA 3

    Plasmid

    #51762

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. ABL1 gRNA (BRDN0001147582)

    Plasmid

    #77732

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ABL1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ABL1 (ABL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge