We narrowed to 3,177 results for: pcdna3 1

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PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

Plasmid

#124493

Purpose

Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipYFP(TEV cleavage seq) T2A mCherry

Use

Synthetic Biology

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

Plasmid

#202606

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-P2A-mCherry-CAAX

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala

Plasmid

#218463

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )

Expression

Mammalian

Mutation

Lys1269Ala and His1271Ala substitutions

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-SF3B1-WT

Plasmid

#82576

Purpose

Expresses a codon-optimized ORF of human SF3B1 (wild-type)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-WT (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

Plasmid

#159130

Purpose

expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nV5-AMOT (p130)

Plasmid

#172997

Purpose

constitutive expression of V5-tagged AMOT (p130)

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOT (p130) (AMOT Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet1 CD

Plasmid

#70129

Purpose

Express flag-tagged mouse Tet1 catalytic domain.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET1 catalytic domain.

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-AKAR4

Plasmid

#181957

Purpose

Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-AKAR4 (AKAP5 Human)

Tags

AKAP79, Cerulean, HIS tag, and cpVenus(E172)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRB-V5/HIS

Plasmid

#201988

Purpose

expression of human PDGFRB receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRB receptor tyrosine kinase (PDGFRB Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RasAR-NES

Plasmid

#205567

Purpose

Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR-NES (RAF1 Synthetic, Human)

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rap1A(WT)

Plasmid

#224276

Purpose

Cell transfection and expression of Rap1A

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rap1A (RAP1A Human)

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 barr1 flag

Plasmid

#14687

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Tags

flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-flag-YTHDF2-C

Plasmid

#52303

Purpose

Flag-tagged YTHDF2 C-terminal domain, from E384 to the end

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Mutation

Deleted amino acids 1-383

Promoter

T7

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cdt1 human

Plasmid

#72681

Purpose

mammalian expression of human Cdt1

Depositor

Domenico Maiorano

Article

Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

Insert

Cdt1 (CDT1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3-ALK2 Q207D

Plasmid

#80871

Purpose

mammalian expression of ALK2 Q207D

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK2 (ACVR1 Human)

Tags

HA

Expression

Mammalian

Mutation

Q207D

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-ALK2 K235R

Plasmid

#80872

Purpose

mammalian expression of ALK2 K235R

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK2 (ACVR1 Human)

Tags

HA

Expression

Mammalian

Mutation

K235R

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

Plasmid

#159132

Purpose

Expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Y11F

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1 K115R

Plasmid

#78835

Purpose

To overexpress RIPK1 K115R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

HA

Expression

Mammalian

Mutation

K115R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-VprBP (C590)

Plasmid

#133589

Purpose

Expresses human VprBP C590 mutant in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

Vpr (HIV-1) binding protein (DCAF1 Human)

Tags

HA

Expression

Mammalian

Mutation

C590 mutation of human VprBP lacking the N-termin…

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-VprBP (RARA)

Plasmid

#133587

Purpose

Expresses human VprBP RARA mutant in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

Vpr (HIV-1) binding protein (DCAF1 Human)

Tags

HA

Expression

Mammalian

Mutation

RARA mutation of human VprBP defective in DDB1 bi…

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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