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We narrowed to 23,602 results for: CRISPR

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Showing: 2011 - 2040 of 23602 results

  3. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  4. pCMU-4

    Plasmid
    #172353
    Purpose
    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA
    Depositor
    Eriko Takano
    Article
    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(
    Insert
    D4-TheoR-cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    D4
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172353-plasmid-map-sequence-id-350007

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  5. pscAAV-Tyr

    Plasmid
    #135616
    Purpose
    Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus
    Depositor
    Lin He
    Article
    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e
    Insert
    EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Available Since
    May 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135616-plasmid-map-sequence-id-296005

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  6. pYPQ290

    Plasmid
    #129670
    Purpose
    Gateway entry clone for CRISPR-Cas12b systems
    Depositor
    Yiping Qi
    Article
    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:
    Insert
    AacCas12b
    Use
    CRISPR; Gateway compatible aaccas12b entry clone
    Expression
    Plant
    Mutation
    AacCas12b is rice codon optimized
    Available Since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    129670-plasmid-map-sequence-id-255066

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  9. pCXLE-dCas9VPPH-T2A-GFP-shP53

    Plasmid
    #102897
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    dCas9VPPH-T2A-GFP-shP53
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102897-plasmid-map-sequence-id-194735

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  11. pPlacPbuCas13b-gRNA-T-MS2

    Plasmid
    #184839
    Purpose
    Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and lac promoter
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    184839-plasmid-map-sequence-id-373965

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  16. pLenti_dCas9-2xAM_hIRF-1

    Plasmid
    #92221
    Purpose
    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Inserts
    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    Use
    CRISPR and Lentiviral
    Tags
    2xAM tag
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    CBh and U6
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    92221-plasmid-map-sequence-id-181086

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  20. pYPQ291

    Plasmid
    #129671
    Purpose
    Gateway entry clone for CRISPR-Cas12b systems
    Depositor
    Yiping Qi
    Article
    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:
    Insert
    BthCas12b
    Use
    CRISPR; Gateway compatible bthcas12b entry clone
    Expression
    Plant
    Mutation
    BthCas12b is rice codon optimized
    Available Since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    129671-plasmid-map-sequence-id-255057

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  21. pPbuCas13b-gRNA-1

    Plasmid
    #184567
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184567-plasmid-map-sequence-id-374002

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  27. pcDNA-LmoCas6-p300

    Plasmid
    #126486
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Insert
    LmoCas6-p300
    Use
    CRISPR
    Tags
    Flag and Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126486-plasmid-map-sequence-id-246633

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  28. pcDNA-CasE-p300-Flag

    Plasmid
    #106275
    Purpose
    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Insert
    EcoCasE-p300
    Use
    CRISPR
    Tags
    E tag, NLS and Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    106275-plasmid-map-sequence-id-247907

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  29. LHZ1493

    Plasmid
    #222102
    Purpose
    Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222102-plasmid-map-sequence-id-441240

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  30. pENTR_AtU6p_BsaI_ccdb_gRNA

    Plasmid
    #225986
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225986-plasmid-map-sequence-id-448026

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Showing: 2011 - 2040 of 23602 results