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We narrowed to 18,759 results for: nls

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Showing: 2681 - 2720 of 18759 results

  1. pcDNA-NLS-ZapCmR1.1

    Plasmid
    #59011
    Purpose
    Nuclear zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Promoter
    pCMV and T7
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  4. pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)

    Plasmid
    #231236
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cells
    Depositor
    Michael Rape
    Publication
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    NLS fused to the DNA-binding-domain of MYC (resid…
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231236-plasmid-map-sequence-id-459169

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  5. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

    Plasmid
    #115142
    Purpose
    CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Publication
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized Mb3Cas12a
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115142-plasmid-map-sequence-id-220502

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  6. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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  8. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

    Plasmid
    #115140
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Publication
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA3 anti-CRISPR protein
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115140-plasmid-map-sequence-id-220495

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  9. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

    Plasmid
    #115139
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Publication
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AcrVA3.1 anti-CRISPR protein
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115139-plasmid-map-sequence-id-220497

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  10. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

    Plasmid
    #115138
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Publication
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA2 anti-CRISPR protein
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115138-plasmid-map-sequence-id-220494

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  12. Kaede-Nucleus-9

    Plasmid
    #57319
    Purpose
    Localization: Nucleus, Excitation: 508 / 572, Emission: 518 / 580
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    Kaede-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  13. pEMS1424

    Plasmid
    #29220
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Publication
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple155-EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Expression
    Mammalian
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    Nov. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
    29220-plasmid-map-sequence-id-13635

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  15. nLYN267-gs10-NLS-VPR

    Plasmid
    #188265
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Publication
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nLYN267 (LYN Human)
    Expression
    Mammalian
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188265-plasmid-map-sequence-id-374533

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  17. NLS-4LNDD-pcDNA3.1(+)

    Plasmid
    #172313
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells
    Depositor
    Joshua Rosenthal
    Publication
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172313-plasmid-map-sequence-id-339585

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  18. Gal4-entry+NLS

    Plasmid
    #128013
    Purpose
    Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer
    Depositor
    Julius Brennecke
    Publication
    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-
    Insert
    Gal4 DBD-SV40NLS-3x-Flag
    Use
    Gal4 entry vector with nls to generate tethering …
    Tags
    Gal4-DBD-SV40NLS-3xFlag
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128013-plasmid-map-sequence-id-251575

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  20. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid
    #114076
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R and K607R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114076-plasmid-map-sequence-id-220531

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  21. pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

    Plasmid
    #133789
    Purpose
    CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CjeCas9
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CAG
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133789-plasmid-map-sequence-id-268569

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  26. dCas9-NLS-gs10-cERb447

    Plasmid
    #188257
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Publication
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cERb447 (ESR1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188257-plasmid-map-sequence-id-374591

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  27. dCas9-NLS-gs10-cTRb413

    Plasmid
    #188261
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Publication
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cTRb413 (TXNRD2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188261-plasmid-map-sequence-id-374572

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  30. mRuby-Nucleus-7

    Plasmid
    #55875
    Purpose
    Localization: Nucleus, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mRuby-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. mPlum-Nucleus-7

    Plasmid
    #55991
    Purpose
    Localization: Nucleus, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mPlum-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. PB-NLS-ZapCV2-ALA

    Plasmid
    #203665
    Purpose
    Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells
    Depositor
    Amy Palmer
    Publication
    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZapCV2-Ala zinc dead sensor
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Cys and His in Zap1 is mutated with Ala
    Promoter
    CMV promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203665-plasmid-map-sequence-id-402878

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Showing: 2681 - 2720 of 18759 results