We narrowed to 3,060 results for: BFP

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pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

Plasmid

#200506

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A and AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44), AAVS1 (ARID1A, AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(23))-PGKpuroBFP-W

Plasmid

#200508

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44), ARID1B(23) (ARID1A, ARID1B Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#200502

Purpose

Lentiviral vector expressing gRNA targeting human AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

AAVS1 (AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

Plasmid

#200503

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43), AAVS1 (SMARCA4, AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W

Plasmid

#200505

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43), SMARCA2(47) (SMARCA4, SMARCA2 Human)

Use

Lentiviral

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199450

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199453

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgT-REX17_EF1a-Puro-T2A-BFP

Plasmid

#195501

Purpose

Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

BFP, Puro, and T2A

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat

Plasmid

#178871

Purpose

Mammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

Plasmid

#167921

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat

Plasmid

#178870

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)

Plasmid

#139237

Purpose

mMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)

Plasmid

#139234

Purpose

mMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)

Plasmid

#139233

Purpose

mMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)

Plasmid

#139232

Purpose

mMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)

Plasmid

#139231

Purpose

mMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

Plasmid

#156404

Purpose

Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Gateway cloning

Tags

mTagBFP2

Mutation

mutant KRAS4A-G12D

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

Plasmid

#128764

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #3 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

Plasmid

#128763

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #2 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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