We narrowed to 2,123 results for: PAM

1. pAAV-CAG-dLight1.2

   Plasmid

   #111069

   Purpose

   To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV1

   Insert

   dLight1.2
   

   Use

   AAV

   Tags

   Flag tag

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LRRK2-K1906M

   Plasmid

   #35946

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-FLAG-MTOR-D2512H

   Plasmid

   #69017

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-D2512H (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Aspartate 2512 to Histidine

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

4. YFP-synDAT-S/A

   Plasmid

   #48792

   Purpose

   Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro

   Depositor

   Jonathan Javitch

   Article

   Fog et al Neuron. 2006 Aug 17;51(4):417-29.

   Insert

   YFP-synhDAT-S/A (SLC6A3 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YFP-synDAT-S/D

   Plasmid

   #48793

   Purpose

   Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro

   Depositor

   Jonathan Javitch

   Article

   Fog et al Neuron. 2006 Aug 17;51(4):417-29.

   Insert

   YFP-synhDAT-S/D (SLC6A3 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5 HA-GST-Presc-mAkt1 (S473T)

   Plasmid

   #48806

   Purpose

   expresses mutant Akt1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   Akt1 (Akt1 Mouse)
   

   Tags

   HA-GST-Presc

   Expression

   Mammalian

   Mutation

   S473T

   Promoter

   CMV

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-TetO(3G)-GCaMP6f-WPRE

   Plasmid

   #186205

   Purpose

   Express GCaMP6f under TetO(3G)

   Depositor

   Naoshige Uchida

   Article

   Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis (N terminal on insert), T7 epitope, and Xpr…

   Promoter

   TetO(3G)

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-mCherry-SynaptoZip

   Plasmid

   #177318

   Purpose

   AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

   Depositor

   Antonio Malgaroli

   Article

   Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

   Insert

   mCherry-SynaptoZip (Vamp2 Rat)
   

   Use

   AAV

   Tags

   Myc and mCherry

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKM33

   Plasmid

   #154167

   Purpose

   Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel2 (long isoform)
   

   Tags

   FLAG

   Expression

   Insect

   Mutation

   C-terminal IκB domain deleted

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJWK_VD_04

    Plasmid

    #158105

    Purpose

    CaV1.3 5'UTR luciferase reporter

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR of CACNA1D (uc003dgu.5)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJWK_VD_03

    Plasmid

    #158104

    Purpose

    CaV1.2 5'UTR luciferase reporter

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR of CACNA1C (uc009zdu.1)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid

    #140001

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid

    #139992

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VRQR=D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

    Plasmid

    #184631

    Purpose

    Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad65 (Gad2 Mouse)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

    Plasmid

    #118273

    Purpose

    pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

    Plasmid

    #139990

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VQR=D1135V/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

    Plasmid

    #139991

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VRER=D1135V/G1218R/R1335E/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-myc-his-BACH1 K52R

    Plasmid

    #17643

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    The lysine residue at position 52 was changed to …

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

20. AAV-hSyn-DIO-mCReP

    Plasmid

    #167503

    Purpose

    Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

    Depositor

    Nicole Calakos

    Article

    Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

    Insert

    Ppp1r15b (Ppp1r15b Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge