We narrowed to 2,098 results for: Pam

1. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

   Plasmid

   #139995

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   HF1=N497A/R661A/Q695A/Q926A

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-DEST51-GOLPH3

   Plasmid

   #21690

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Invitrogen gateway destination vector

   Tags

   6xHIS and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

   Plasmid

   #118273

   Purpose

   pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis (N terminal on insert), T7 epitope, and Xpr…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5_FKBP25-V5-APEX2

   Plasmid

   #83418

   Purpose

   Expressed FKBP25/FKBP3-V5-APEX2 in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

   Insert

   Peptidyl-prolyl cis-trans isomerase FKBP3 (FKBP3 Human)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-flex-TVA

   Plasmid

   #69618

   Purpose

   Express recepter TVA for envA with AAV

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

   Insert

   Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-HIF2α P405A;P531A;bHLH*-pBabe-Puro

   Plasmid

   #22135

   Depositor

   William Kaelin

   Article

   Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids from wt: Proline 405 t…

   Promoter

   Available since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMS-eGFP-RTP801

   Plasmid

   #65057

   Purpose

   Expression of RTP801 in mammalian cells

   Depositor

   Cristina Malagelada, Lloyd Greene

   Article

   Malagelada et al J Neurosci. 2006 Sep 27;26(39):9996-1000

   Insert

   DDIT4 (Ddit4 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pS6-hα2M-H6

   Plasmid

   #128495

   Purpose

   Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (G27-S1468) (A2M Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIE-hα2M-H6

   Plasmid

   #128492

   Purpose

   Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (A2M Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Insect

   Mutation

   Promoter

   IE1

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJWK_VD_01

    Plasmid

    #158102

    Purpose

    5'UTR stem-loop luciferase reporter: 0kcal/mol

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR synthetic stem-loop 0kcal/mol
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pS6-C-hα2M-H6

    Plasmid

    #128497

    Purpose

    Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (F789-S1468) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJWK_VD_02

    Plasmid

    #158103

    Purpose

    5'UTR stem-loop luciferase reporter: -30kcal/mol

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR synthetic stem-loop -30kcal/mol
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-GOLPH3

    Plasmid

    #21688

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    last nucleotide of stop codon removed for c-termi…

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pLV.tetO.lmx1a

    Plasmid

    #234847

    Purpose

    3rd generation lentiviral vector, expresses Lmx1a under control of TetON promoter

    Depositor

    Malin Parmar

    Article

    Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

    Insert

    Lmx1a (Lmx1a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIH FRB-BFP

    Plasmid

    #202425

    Purpose

    TagBFP FKBP-rapamycin binding (FRB) dimerization domain

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    FKBP-rapamycin binding (FRB) dimerization domain
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

    Plasmid

    #184636

    Purpose

    Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Gat1 gene with Arginine 69 changed to Lysine, to …

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIE-N-hα2M-H6

    Plasmid

    #128493

    Purpose

    Expresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (S24-E908) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    Promoter

    IE1

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pS6-N-hα2M-H6

    Plasmid

    #128496

    Purpose

    Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (G27-A788) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMCh-695

    Plasmid

    #92263

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 PAM2 mut (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced: EF…

    Promoter

    Ac5

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-AKAP95-FRB

    Plasmid

    #138254

    Purpose

    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    AKAP95-FRB (AKAP8 Human)
    

    Use

    Tags

    FRB rapamycin binding domain and HA epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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