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pMRX-IB-HaloTag7-mGFP

Plasmid

#184903

Purpose

Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

monomeric EGFP

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHN924

Plasmid

#178885

Purpose

Expression of GST-His8-HRV3C

Depositor

Kazuhiro Abe

Insert

human rhinovirus 3C protease

Tags

GST, His8

Expression

Bacterial

Promoter

T7

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176272

Purpose

Expression of non-targeting sgRNA of CasMINI

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI non-targeting sgRNA (sgLacZ) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pet-15b-ultraID

Plasmid

#172879

Purpose

expresses ultraID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Article

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_256

Plasmid

#158581

Purpose

Lentiviral expression of BE3.9max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-FOXO1A

Plasmid

#153141

Purpose

Expression of Flag tagged human FOXO1A in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXO1 (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_DA2h

Plasmid

#140554

Purpose

Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

The 2nd generation DA sensor GRAB_DA2h (high affinity)

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

Plasmid

#138461

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-VP64-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP10

Plasmid

#141264

Purpose

Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 3, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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