We narrowed to 5,913 results for: pcDNA 3.1

1. pcDNA3.1/NDUFS7/myc-His

   Plasmid

   #127488

   Purpose

   Expresses myc-tagged NDUFS7 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS7 (NDUFS7 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-MS2-HA-Hs4E-T_AA

   Plasmid

   #148233

   Purpose

   Mammalian Expression of Hs4E-T

   Depositor

   Elisa Izaurralde

   Insert

   Hs4E-T (EIF4ENIF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LARG mCherry sspB in pcDNA3.1

   Plasmid

   #90459

   Purpose

   RhoA mediated cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Inserts

   LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
   mCherry
   sspB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R73Q

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Clover-Prx2-mRuby2

   Plasmid

   #117906

   Purpose

   fluorescent reporter of cytosolic hydrogen peroxide

   Depositor

   Hadley Sikes

   Article

   Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

   Insert

   Clover-Prx2-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-SARS-CoV-2-S (D614G)

   Plasmid

   #215838

   Purpose

   Expresses SARS-CoV-2 Spike protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1 (-) + codon optimized Brn3a

   Plasmid

   #86829

   Purpose

   mammalian expression of the transcription factor Brn3a

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   Brn3a (POU4F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 Flag Runx1 FL (P#1790)

   Plasmid

   #14585

   Depositor

   Matija Peterlin

   Article

   Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

   Insert

   Runx1 (Runx1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/GUG-DAP5-3XFLAG

   Plasmid

   #127330

   Purpose

   Express 3xFLAG tagged DAP5 protein from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   DAP5 (EIF4G2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. Arf6 T27N- Venus in pcDNA3.1

    Plasmid

    #90464

    Purpose

    RhoA mediated cell migration

    Depositor

    Narasimhan Gautam

    Article

    Castillo-Badillo et al ACS Synth Biol. 2020 Mar 16. doi: 10.102

    Insert

    Arf6 T27N (ARF6 )
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/Myc-His(-)-MSulf-2

    Plasmid

    #13008

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    mouse sulfatase 2 (Sulf2 Mouse)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-N-Myc_1-205 USP7

    Plasmid

    #131245

    Purpose

    Mammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses a mutant form of our pcDNA3.1-N-Myc_1-5…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1/NDUFS2/myc-His

    Plasmid

    #127486

    Purpose

    Expresses myc-tagged NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid

    #128746

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 point mutations disrupting the miR-7 site

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+CMV-Ins-GLuc

    Plasmid

    #89929

    Purpose

    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

    Depositor

    Melanie Cobb

    Article

    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

    Insert

    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DNA sequence for Gaussia luciferase was humanized…

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1his p66shc S36A (corrected)

    Plasmid

    #32575

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    S36A

    Promoter

    CMV

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 SARS-CoV-2 S D614G

    Plasmid

    #158075

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-N-Myc_1-560 USP7

    Plasmid

    #131244

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses USP7 amino acids 1 - 560. This truncati…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MS2CP-VPg(FCV)

    Plasmid

    #167314

    Purpose

    Template pDNA to prepare CaVT mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    CaVT
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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