Addgene

We narrowed to 22,251 results for: Sele

Showing: 2041 - 2070 of 22251 results

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pGADT7 Hsh155 K335T

Plasmid

#87300

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335T

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335T

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 K335N

Plasmid

#87298

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335N

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335N

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 K335E

Plasmid

#87297

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335E

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 R294C

Plasmid

#87292

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 R294C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

R294C

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 E291D

Plasmid

#87290

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 E291D

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

E291D

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/K335E

Plasmid

#87286

Purpose

Encodes Hsh155 N295D K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

N295D & K335E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/D450G

Plasmid

#87284

Purpose

Encodes Hsh155 R294L D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

R294L & D450G

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/K335E

Plasmid

#87283

Purpose

Encodes Hsh155 R294L K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

R294L & K335E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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Ubiquitin K6C/G76C

Plasmid

#86584

Purpose

Bacterial expression of Ubiquitin K6C/G76C

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K6C/G76C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Ubiquitin K48C/G76C

Plasmid

#86585

Purpose

Bacterial expression of Ubiquitin K48C/G76C

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K48C/G76C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Ubiquitin K63C/G76C

Plasmid

#86586

Purpose

Bacterial expression of Ubiquitin K63C/G76C

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K63C/G76C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB233

Plasmid

#68624

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

MpHSP17.8A1 promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB210

Plasmid

#68601

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Plant

Mutation

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119

Plasmid

#66136

Purpose

Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(T214V)

Plasmid

#66137

Purpose

Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 T180C

Plasmid

#66181

Purpose

Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Use

Tags

His6 tag

Expression

Bacterial

Mutation

T180C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 R112C

Plasmid

#66180

Purpose

Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Use

Tags

His6 tag

Expression

Bacterial

Mutation

R112C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C

Plasmid

#66176

Purpose

Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E105C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(D77R/T214V)

Plasmid

#66138

Purpose

Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA5woH

Plasmid

#65019

Purpose

Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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SI13-Lrrc3b

Plasmid

#46303

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 . 8():76.

Insert

Lrrc3b mouse monoclonal antibody (low affinity) (lrrc3b Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pQTEV-PTK9L

Plasmid

#31587

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

PTK9L protein tyrosine kinase 9-like (A6-related protein) (TWF2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pNH066

Plasmid

#42162

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP Tag-RT-::mTFP1

Use

Tags

TAP-TAg

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH009

Plasmid

#42146

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP-tag::mTFP1

Use

Tags

TAP-Tag

Expression

Worm

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-neo

Plasmid

#21916

Purpose

3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

Depositor

Dmitri Wiederschain

Article

Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-C1-PLCdelta-PH

Plasmid

#21179

Purpose

encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

Depositor

Tobias Meyer

Article

Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

Insert

PLCdelta (PLCD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

PH domain of human PLC delta, amino acids Met1–Il…

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV SCH9

Plasmid

#212708

Purpose

A SCHEMA evolved AAV variant effective at targeting subventricular NSCs

Depositor

David Schaffer

Article

Ojala et al Mol Ther. 2018 Jan 3;26(1):304-319. doi:

Insert

AAV2 Rep SCH9 Cap

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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loxP-G418-LoxP-TurboID-Rab11 HR

Plasmid

#230026

Purpose

Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

Depositor

Francesca Bottanelli

Insert

genomic sequence Rab11 locus (RAB11A Human)

Use

CRISPR

Tags

Turbo-ID, V5

Expression

Mutation

Promoter

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-MCS--4x6T-WPRE

Plasmid

#196417

Purpose

Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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