Addgene

We narrowed to 11,705 results for: pcDNA

Showing: 3401 - 3450 of 11705 results

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pcDNA3-hRPL3-FLAG_WT

Plasmid

#233616

Purpose

Expression of Flag-tagged hRPL3

Depositor

Shintaro Iwasaki

Article

Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

Insert

Flag-tagged hRPL3 (RPL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hRPL3-FLAG_H245A

Plasmid

#233617

Purpose

Expression of Flag-tagged hRPL3 with H245A mutation

Depositor

Shintaro Iwasaki

Article

Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

Insert

Flag-tagged hRPL3 with H245A mutation (RPL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H245A

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag-TUBG2

Plasmid

#229855

Purpose

TUBG2

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

Insert

TUBG2 (TUBG2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Removal of the internal TUBG2-EcoRI site by chang…

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-SP1

Plasmid

#232649

Purpose

expresses SP1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP1 (SP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 N-T2A-M-IRES-E

Plasmid

#231903

Purpose

For the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' system

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, M, E SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

R203M in N protein

Promoter

CMV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FFSS-BACH1

Plasmid

#232273

Purpose

Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-BACH2

Plasmid

#232275

Purpose

Expression of FLAG-tagged BACH2 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH2 (BACH2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1a-FLAG

Plasmid

#232143

Purpose

Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1a (dgat1a Zebrafish)

Use

Zebrafish expression

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ mogat3b-FLAG

Plasmid

#232145

Purpose

Expresses zebrafish mogat3b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3b

Use

Zebrafish expression

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sga-Cas9

Plasmid

#227005

Purpose

Mammalian expression of Nuclease active S. gallolyticus Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sin-Cas9

Plasmid

#227006

Purpose

Mammalian expression of Nuclease active S. iniae Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Spa-Cas9

Plasmid

#227007

Purpose

Mammalian expression of Nuclease active S. parasanguinis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sub-Cas9

Plasmid

#227008

Purpose

Mammalian expression of Nuclease active S. uberis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-hMAPT

Plasmid

#231583

Purpose

Minigene of human MAPT containing exon 9, 10, 11 and relevant intronic sequences

Depositor

Chaolin Zhang

Article

Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

Insert

human MAPT (MAPT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nmEML-mRFP

Plasmid

#231178

Purpose

Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-15nmEML-mRFP

Plasmid

#231177

Purpose

Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

15nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, K98A)

Plasmid

#231377

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

H56A, K98A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A, K98A)

Plasmid

#231378

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R97A, K98A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97E, K98E)

Plasmid

#231380

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R97E, K98E

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A, H712A)

Plasmid

#231383

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R710A, H712A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-F-box:gs10:FRB:HA

Plasmid

#228531

Purpose

F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

F-box:gs10:FRB:HA (SKP2 Human, Synthetic)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S724A

Plasmid

#224735

Purpose

For expression of HA-tagged GTSE1 protein with S724A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 724 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S734A

Plasmid

#224736

Purpose

For expression of HA-tagged GTSE1 protein with S734A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 734 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S171A

Plasmid

#224723

Purpose

For expression of HA-tagged GTSE1 protein with S171A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 171 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S187A

Plasmid

#224724

Purpose

For expression of HA-tagged GTSE1 protein with S187A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 187 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S262A

Plasmid

#224725

Purpose

For expression of HA-tagged GTSE1 protein with S262A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 262 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S345A

Plasmid

#224726

Purpose

For expression of HA-tagged GTSE1 protein with S345A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 345 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S417A

Plasmid

#224727

Purpose

For expression of HA-tagged GTSE1 protein with S417A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 417 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S480A

Plasmid

#224728

Purpose

For expression of HA-tagged GTSE1 protein with S480A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 480 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S555A

Plasmid

#224729

Purpose

For expression of HA-tagged GTSE1 protein with S555A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 555 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S594A

Plasmid

#224730

Purpose

For expression of HA-tagged GTSE1 protein with S594A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 594 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S599A

Plasmid

#224731

Purpose

For expression of HA-tagged GTSE1 protein with S599A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 599 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S611A

Plasmid

#224732

Purpose

For expression of HA-tagged GTSE1 protein with S611A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 611 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S707A

Plasmid

#224733

Purpose

For expression of HA-tagged GTSE1 protein with S707A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 707 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S718A

Plasmid

#224734

Purpose

For expression of HA-tagged GTSE1 protein with S718A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 718 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S84A

Plasmid

#224720

Purpose

For expression of HA-tagged GTSE1 protein with S84A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 84 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S91A

Plasmid

#224721

Purpose

For expression of HA-tagged GTSE1 protein with S91A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 91 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S157A

Plasmid

#224722

Purpose

For expression of HA-tagged GTSE1 protein with S157A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 157 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/V5-His-mPer1

Plasmid

#219421

Purpose

Expresses 3873 bp of mPer1 coding sequence in E. coli.

Depositor

Aziz Sancar

Article

Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mPer1 (Per1 Mouse)

Use

Tags

His and V5

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Nuc-deltaCKAR

Plasmid

#222430

Purpose

FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Nucleus delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Mito(OM)-deltaCKAR

Plasmid

#222431

Purpose

FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Mitochondrial outer membrane delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Golgi-deltaCKAR

Plasmid

#222428

Purpose

FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Golgi delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
His-Precission-NEDD4L_C942S_pCDNA3.1(+)

Plasmid

#187729

Purpose

Express the catalytically inactive NEDD4L FL in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

Cys874 mutated to a serine

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
His-Precission-NEDD4L_FL_pCDNA3.1(+)

Plasmid

#187730

Purpose

Express NEDD4L FL in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_SM-FLuc0-malat1

Plasmid

#210580

Purpose

Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_SM-FLuc100-malat1

Plasmid

#210581

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2D-A

Plasmid

#221734

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D683A in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2DY-EF

Plasmid

#221733

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E and Y680F in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2Y-F

Plasmid

#221732

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Y680F in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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