We narrowed to 4,292 results for: NAP

1. pDC184

   Plasmid

   #70055

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with p045

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   His tagged Synaptobrevin 28-89
   Syntaxin 191-256
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CVS-N2c-EGFP-FlpO

   Plasmid

   #172373

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   EGFP + FlpO
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CVS-N2c-jGCaMP8f

   Plasmid

   #172387

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   GCaMP8f
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CVS-N2c-nl.EGFP-tdTomato

   Plasmid

   #172382

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized EGFP + tdTomato
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. RabV CVS-N2c(deltaG)-APEX2-Venus

   Plasmid

   #73467

   Purpose

   Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   APEX2-Venus
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET51b-Strep-SsOGT-H5-His

   Plasmid

   #167277

   Purpose

   Overexpression of a sulfolobal equivalent of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SsOGT-H5
   

   Use

   Tags

   His x10 and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 8xCTS1_BpiI-cloning-site_SV40polyA

   Plasmid

   #124547

   Purpose

   Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.0_nucLAMA-G97 IRES mYFP

   Plasmid

   #130713

   Purpose

   Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-LAMA-G97-NLS IRES mYFP
   

   Use

   Tags

   NLS and SNAP-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.0_nucLAMA-F98 IRES mYFP

   Plasmid

   #130712

   Purpose

   Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-LAMA-F98-NLS IRES mYFP
   

   Use

   Tags

   NLS and SNAP-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.0_mitoLAMA-G97 IRES EGFP

    Plasmid

    #130707

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-G97 IRES EGFP
    

    Use

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC171

    Plasmid

    #118888

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease; SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDD2024

    Plasmid

    #101368

    Purpose

    Name: pCBG68-RBPjkΔA. RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    RBPjk binding site A mutation in pCBG68-LANApi (p…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDD2030

    Plasmid

    #101373

    Purpose

    Name: pBluescript-RBPjkΔB. RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    RBPjk binding site B mutation in pBluescript-LANA…

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDD2031

    Plasmid

    #101374

    Purpose

    Name: pBluescript-RBPjkΔA. RBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    RBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    RBPjk binding site A mutation in pBluescript-LANA…

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGMCK-0X750-luxAB-DAS+4

    Plasmid

    #49517

    Purpose

    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGMEH-0X750-luxAB-DAS+4

    Plasmid

    #49519

    Purpose

    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomally

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDD2013

    Plasmid

    #101364

    Purpose

    Name: pCBG68-K14ΔTATA. K14 TATA box mutation in pCBG68-LANApi (pDD2002).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    K14 TATA box mutation in pCBG68-LANApi (pDD2002).
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    K14 TATA box mutation in pCBG68-LANApi (pDD2002).

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD2022

    Plasmid

    #101366

    Purpose

    Name: pCBG68-RBPjkΔB. RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    RBPjk binding site B mutation in pCBG68-LANApi (p…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pN565

    Plasmid

    #49990

    Purpose

    expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    T7 RNAP*
    

    Use

    Synthetic Biology

    Tags

    umuD degradation tag

    Expression

    Bacterial

    Mutation

    R632S

    Promoter

    pTac-symmetric

    Available since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMX_mTBP

    Plasmid

    #44333

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TBP (Tbp Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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