We narrowed to 2,422 results for: NIG

1. NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75237

   Purpose

   CRISPR/Cas9 plasmid against human NFATc1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK mCh-V5-NES-EGFP-ERBB2 NLS zeo

   Plasmid

   #192338

   Purpose

   Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgSf3b1(T1)

   Plasmid

   #90424

   Purpose

   induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700

   Depositor

   Alex Minella

   Article

   Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

   Insert

   mus Sf3b1 gRNA (Sf3b1 Mouse)
   

   Use

   CRISPR; Guiderna encoding for gene editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIG-hNFATc1/bC - AVITEV

   Plasmid

   #74059

   Purpose

   retroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc1, isoform beta-C (NFATC1 Human)
   

   Use

   Retroviral

   Tags

   AVI-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   pMSCV-LTRs

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-VQRRA-P2A-GFP-PGK-Puro

   Plasmid

   #110864

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-3xNLS-OptoJNKi-dsm

   Plasmid

   #89743

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi dark-state mutant
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C450A mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-3xNLS-OptoJNKi-lsm

   Plasmid

   #89742

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi lit-state mutant
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   I539E mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-VRERRA-P2A-GFP-PGK-Puro

   Plasmid

   #110865

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBI121::smEclipGFP:AQ

   Plasmid

   #240454

   Purpose

   Expression of indicator protein fusion (soluble modified ecliptic pHluorin & Aequorin) for monitoring calcium concentrations and pH in the cytoplasm of higher plants. For details, see Comments.

   Depositor

   Christoph Plieth

   Article

   Gao et al Plant Physiol. 2004 Mar;134(3):898-908.

   Insert

   Fusion of soluble modified ecliptic pHluorin and Aequorin
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Ecliptic GFP (ecliptic pHluorin) (PMID 9671304); …

   Promoter

   Cauliflower mosaic virus 35S promoter (GenBank AJ…

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBI121::smRatioGFP:AQ

    Plasmid

    #240453

    Purpose

    Expression of indicator protein fusion (soluble modified ratiometric pHluorin & Aequorin) for monitoring calcium concentrations and pH in the cytoplasm of higher plants. For details, see Comments.

    Depositor

    Christoph Plieth

    Article

    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.

    Insert

    Fusion of soluble modified ratiometric pHluorin and Aequorin
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Ratiometric GFP (ratiometric pHluorin) (PMID 9671…

    Promoter

    Cauliflower mosaic virus 35S promoter (GenBank AJ…

    Available since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet_6xHis-FKBP8(1-391aa)-thrombin-GST

    Plasmid

    #227712

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    FKBP8(1-391aa) (FKBP8 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet_6xHis-CCPG1(1-212aa)-thrombin-GST

    Plasmid

    #227713

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    CCPG1(1-212aa) (CCPG1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIP-SFFV-EGFP-FLAG-UL36USP (C40)

    Plasmid

    #235548

    Purpose

    To express GFP-FLAG-UL36USP (C40) - region 25-520 of UL36

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    UL36USP (C40)
    

    Use

    Lentiviral

    Tags

    EGFP-FLAG

    Expression

    Mutation

    N-terminal part of UL36 starting at the second AT…

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIP-SFFV-EGFP-FLAG-UL36USP (C65)

    Plasmid

    #235549

    Purpose

    To express GFP-FLAG-UL36USP (C65) - region 1-520 of UL36

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    UL36USP (C65)
    

    Use

    Lentiviral

    Tags

    EGFP-FLAG

    Expression

    Mutation

    N-terminal part of UL36 starting at the first ATG…

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1-puro-USP7-sh1

    Plasmid

    #235527

    Purpose

    shRNA against human USP7

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    USP7 (USP7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-puro-USP7-sh5

    Plasmid

    #235528

    Purpose

    shRNA against human USP7

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    USP7 (USP7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-MBP-OSF-TEVcs-GFP-hULK1

    Plasmid

    #239015

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    hULK1 (ULK1 Human)
    

    Use

    Tags

    MBP-OSF-TEV-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGenLenti V5-NIX (W36A/L39A; deltaLIR)

    Plasmid

    #223788

    Purpose

    Stable expression of protein in mammalian cell culture

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    NIX (BNIP3L) (BNIP3L Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    W36A/L39A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG_mCherry-ATG13 HORMA (1-191aa)

    Plasmid

    #223759

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    ATG13 HORMA (ATG13 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 1-191 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

    Plasmid

    #223764

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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