We narrowed to 2,366 results for: clu

1. GCN5 FL-dCas9-CBP core

   Plasmid

   #179556

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GCN5 core-dCas9-CBP core

   Plasmid

   #179555

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CBP core-dCas9-p300 core

   Plasmid

   #179558

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) & c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GCN5 FL-dCas9-p300 core

   Plasmid

   #179554

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR221-TAF15 g1172a No Stop

   Plasmid

   #84898

   Purpose

   DONOR vector for Gateway cloning of TAF15 g1172a No Stop

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Gateway donor

   Tags

   Expression

   Mutation

   g1172a No Stop

   Promoter

   none

   Available since

   April 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-TetOminiCMV-OSKM

   Plasmid

   #136541

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOME0295M

   Plasmid

   #182624

   Purpose

   KSHV Reverse Sequence Negative control Kaposinclouster_RevCompl-KapATGdel_Syn

   Depositor

   Dirk Dittmer

   Insert

   KSHV Kaposincluster_RevCompl_KapATGdel_SYN_rev
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUB2.1

   Plasmid

   #40728

   Purpose

   Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background

   Depositor

   Thomas Hughes

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAST-3x attB

    Plasmid

    #220329

    Purpose

    includes 3 standard UAS cassettes

    Depositor

    Erdem Bangi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid

    #101893

    Purpose

    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti Lifeact-mRuby2 PuroR

    Plasmid

    #84386

    Purpose

    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLfa[Tc'hsp5'3'UTR]fa

    Plasmid

    #86450

    Purpose

    Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)

    Depositor

    Gregor Bucher

    Insert

    Tc'hsp5'3'UTR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET14b_tev_PDZ1

    Plasmid

    #59739

    Purpose

    expresses PDZ1 domain of human ZO-1 in bacteria

    Depositor

    Alan Fanning

    Insert

    zonula occludens-1 (TJP1 Human)
    

    Use

    Tags

    HIS tag and thrombin clvg

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCI-SMN2-skipping-GLuc

    Plasmid

    #218670

    Purpose

    Alternative splicing signaling: signal decrease with Ex7 inclusion increasing

    Depositor

    Jingxin Wang

    Insert

    SMN2 (SMN2 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKP1469

    Plasmid

    #160815

    Purpose

    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)

    Depositor

    Kathleen Postle

    Insert

    exbB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

    Plasmid

    #84388

    Purpose

    Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. 2xUAS-1xhsp70-attB

    Plasmid

    #220317

    Purpose

    includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette

    Depositor

    Erdem Bangi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR CMVie-Lifeact-mCardinal L1-R5

    Plasmid

    #84392

    Purpose

    Multisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK531

    Plasmid

    #72512

    Purpose

    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)

    Depositor

    T. J. Kappock

    Insert

    hypothetical protein in purSQL operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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