We narrowed to 15,399 results for: nans

1. sgKRAS(B)

   Plasmid

   #85567

   Purpose

   Expresses sgRNA targeting human KRAS around codon G12

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   KRAS proto-oncogene, GTPase (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

2. sgKRAS(A)

   Plasmid

   #85566

   Purpose

   Expresses sgRNA targeting human KRAS around codon G12

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   KRAS proto-oncogene, GTPase (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

3. FUW-tetO-hOKMminiSOX

   Plasmid

   #206383

   Purpose

   Expresses four genes (human OCT4, KLF4, c-MYC and mouse miniSOX) in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   OCT4, KLF4, c-MYC, miniSOX (POU5F1, KLF4, MYC Human, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

   Plasmid

   #185491

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHIS-MDH1-HIS

   Plasmid

   #184558

   Purpose

   Bacterial expression of human MDH1 with HIS Tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Insert

   Malate dehydrogenase 1 (MDH1 Human)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

   Plasmid

   #185488

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-P2A-eGFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

   Plasmid

   #185485

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-P2A-eGFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

   Plasmid

   #185490

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

   Plasmid

   #162878

   Purpose

   Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)

    Plasmid

    #185484

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441)

    Plasmid

    #185486

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380)

    Plasmid

    #185487

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid

    #185489

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid

    #185492

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FUW-tetO-hOKMSOX17FNV

    Plasmid

    #206382

    Purpose

    Expresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    OCT4, KLF4, c-MYC, SOX17FNV (POU5F1, KLF4, MYC, SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

    Plasmid

    #184549

    Purpose

    Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Inserts

    Malate dehydogenase 1 (MDH1 Human)
    Malic Enzyme 1 (ME1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG and HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUltra-MDH1-ME1

    Plasmid

    #184465

    Purpose

    Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Inserts

    Malate dehydrogenase 1 (MDH1 Human)
    Malic Enzyme 1 (ME1 Human)
    

    Use

    Lentiviral

    Tags

    fused to T2A

    Expression

    Mammalian

    Mutation

    deletion of stop codon

    Promoter

    UbC

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pR2HYG

    Plasmid

    #30170

    Depositor

    Lalita Ramakrishnan

    Insert

    Mycobacterium Strong Promoter (MSP)
    

    Use

    Mycobacteria expression

    Tags

    dsRed2

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti NbALFA-miRFP680 hygro

    Plasmid

    #193969

    Purpose

    lentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, hygromycin resistance

    Depositor

    Raphael Gaudin

    Insert

    NbALFA-miRFP680
    

    Use

    Lentiviral

    Tags

    miRFP680

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti NbALFA-miRFP680 puro

    Plasmid

    #193972

    Purpose

    lentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, puromycin resistance

    Depositor

    Raphael Gaudin

    Insert

    NbALFA-miRFP680
    

    Use

    Lentiviral

    Tags

    miRFP680

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge