We narrowed to 26,837 results for: tat

1. pCDF1-PTPRD-D1248N

   Plasmid

   #25646

   Depositor

   Todd Waldman

   Article

   Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

   Insert

   Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   tumor-derived mutation created by site-directed m…

   Promoter

   Available since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pTC362

   Plasmid

   #91216

   Purpose

   protoplast vector for targeted deletion of 6 genes in tomato

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNAs targeting 6 tomato genes
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRN3P-HA-Suv4-20h2mut

   Plasmid

   #86692

   Purpose

   For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

   Insert

   histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)
   

   Use

   Tags

   HA

   Expression

   Mutation

   Mutated asparagine 273 to cysteine 276 (NHDC) int…

   Promoter

   T3

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-ADAM7 (H243Y)

   Plasmid

   #31138

   Depositor

   Yardena Samuels

   Article

   Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

   Insert

   ADAM7 (ADAM7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Histidine 243 changed to Tyrosine

   Promoter

   Available since

   Sept. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pk335-CARGO-6mer-51kb-DSF

   Plasmid

   #227496

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 51kb Downstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pk335-CARGO-6mer-70kb-DSF

   Plasmid

   #227498

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 70kb Downstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pk335-CARGO-6mer-26kb-USF

   Plasmid

   #227467

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 26kb Upstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBSII-PGK-FLPo-IRES-Puro

   Plasmid

   #205989

   Purpose

   Expresses Flp recombinase and puromycin resistance

   Depositor

   Sharon Torigoe

   Article

   Waite et al PLoS One. 2024 Sep 30;19(9):e0311120. do

   Inserts

   PGK promoter
   FLPo (flp )
   IRES-PuroR cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

   Plasmid

   #207054

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   L55W T56A V61W R65A

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2/shCol1a1/Scarlet-Seq1.1

    Plasmid

    #201401

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2/Col1a1/GFP4_Seq 1.1

    Plasmid

    #201400

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDA2ScIfm

    Plasmid

    #193786

    Purpose

    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Frame-shifted CI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTATAA synthetic promoter carrying binding sites…

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKIF1C_P176L-mCherry-FRB

    Plasmid

    #185983

    Purpose

    Mammalian expression of human KIF1C with P176L mutation and mCherry and FRB tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    mCherry and FRB

    Expression

    Mammalian

    Mutation

    P176L - mutation causing HSP (SPG58)

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac-M13-6His-KIF1C_R169W-GFP

    Plasmid

    #185979

    Purpose

    Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    6xHis and eGFP

    Expression

    Insect

    Mutation

    R169W - mutation causing HSP (SPG58)

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac-M13-6His-KIF1C_P176L-GFP

    Plasmid

    #185978

    Purpose

    Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    6xHis and eGFP

    Expression

    Insect

    Mutation

    P176L - mutation causing HSP (SPG58)

    Promoter

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hy_pMT Flag-URH49 MUT

    Plasmid

    #110126

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 amino acid mutations (RSFS motif changed to KSL…

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hy_pMT Flag-Hel25E MUT

    Plasmid

    #110122

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 mutations (KKLN motif changed to RSFS)

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCHKO_HPRT1_exon3_1_As

    Plasmid

    #155057

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid

    #155053

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCEP4-001428(753)-VC

    Plasmid

    #123256

    Purpose

    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation fused to the C-terminal half of split fluorescent Venus (VC)

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (001428) Env
    

    Use

    Tags

    CD5 leader peptide and VC (Venus residues D155–K2…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; C-terminal Env re…

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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