1,862 results

1. pCFJ421 - Pmyo-2::GFP::H2B (pharynx)

   Plasmid

   #34876

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   Pmyo-2 GFP H2B tbb-2 UTR
   

   Use

   Worm targeting

   Tags

   Expression

   Mutation

   S65C

   Promoter

   myo-2

   Available since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

   Plasmid

   #107939

   Purpose

   pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   mCherry(dpiRNA)::ANI-1(AHPH)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   pie-1

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFJ1415

   Plasmid

   #84828

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Peft-3

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. coel::GFP

   Plasmid

   #8937

   Depositor

   Piali Sengupta

   Article

   Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

   Insert

   unc-122 promoter (unc-122 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM320 - sgRNA mosTI unc-119 array insertion

   Plasmid

   #159824

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 array insertion
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a Ce-iPGM-bio

   Plasmid

   #162559

   Purpose

   Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   C. elegans iPGM bio-6His (ipgm-1 Nematode)
   

   Use

   Tags

   6His tag, T7 tag, Thrombin cleavage site, and bio…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGLOW31

   Plasmid

   #172698

   Purpose

   C. elegans mScarlet co-injection marker

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   Pmyo-3::mScarlet
   

   Use

   Tags

   mScarlet

   Expression

   Worm

   Mutation

   Promoter

   myo-3

   Available since

   Aug. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pJR014

   Plasmid

   #198813

   Purpose

   Natural light-gated anion channels. In vivo inhibition of neurons using blue light.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GtACR2::SL2::GFP::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJW2171

   Plasmid

   #163095

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 3xHA-miniTurbo_pAS32

    Plasmid

    #118222

    Purpose

    expresses 3xHA-tagged miniTurbo in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    ges-1p

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR

    Plasmid

    #225924

    Purpose

    Expressing LGC-47 in AVA neuron

    Depositor

    Andrew Leifer

    Article

    Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

    Insert

    LGC-47 (lgc-47 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rig-3

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMS178

    Plasmid

    #215678

    Purpose

    Empty repair plasmid for ChrII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxP + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMS114

    Plasmid

    #215677

    Purpose

    Empty repair plasmid for ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxN + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCZGY405

    Plasmid

    #172694

    Purpose

    Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    rab-3 (rab-3 Nematode)
    

    Use

    Tags

    Punc-25 and mcherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mg319

    Plasmid

    #73367

    Purpose

    unc-70 tension sensor

    Depositor

    Miriam Goodman

    Article

    Krieg et al Nat Cell Biol. 2014 Mar;16(3):224-33. do

    Insert

    unc-70::TSMod
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    unc-70p

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW6.01

    Plasmid

    #62882

    Purpose

    Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    GFP::MEG-3
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    meg-3

    Available since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ1320

    Plasmid

    #84829

    Purpose

    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized GFP
    

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Peft-3

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXF121

    Plasmid

    #139028

    Purpose

    MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    short mex-5 promoter (mex-5 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pJJR83

    Plasmid

    #75028

    Purpose

    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    mCherry^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge