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Showing: 301 - 350 of 613 results

  1. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162682-plasmid-map-sequence-id-320836

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  3. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162673-plasmid-map-sequence-id-321521

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  4. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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  5. pCJ220

    Plasmid
    #162686
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224W, C529S; codon optimized for…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162686-plasmid-map-sequence-id-321468

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  6. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162687-plasmid-map-sequence-id-321457

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  7. pOPINB-HOIL-1 helix-RBR (233–510)

    Plasmid
    #193859
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Residues 233–510, codon optimised for expression …
    Promoter
    T7
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193859-plasmid-map-sequence-id-379616

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  8. pcDNA3 HA-humanTRAF2 (1084)

    Plasmid
    #66932
    Purpose
    Mammalian Expression of TRAF2
    Depositor
    Michael Karin
    Insert
    TRAF2 (TRAF2 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    66932-plasmid-map-sequence-id-190248

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  9. pcDNA3 Flag-humanTRAF2 (1087)

    Plasmid
    #66931
    Purpose
    Mammalian Expression of TRAF2
    Depositor
    Michael Karin
    Insert
    TRAF2 (TRAF2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    66931-plasmid-map-sequence-id-190246

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  10. pcDNA3-TRAM-CFP

    Plasmid
    #13027
    Depositor
    Doug Golenbock
    Insert
    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13027-plasmid-map-sequence-id-7425

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  12. pcDNA3-Xiap-Myc K322/328R

    Plasmid
    #11834
    Depositor
    Guy Salvesen
    Insert
    XIAP K322/328R (XIAP Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    K322/328R
    Promoter
    Available since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only

  13. pSil-XIAP

    Plasmid
    #58832
    Purpose
    Express human apoptosis inhibitor XIAP from CAG promoter
    Depositor
    Troy Margrie
    Insert
    XIAP (XIAP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    58832-plasmid-map-sequence-id-91146

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  14. SEC14L4

    Plasmid
    #73233
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEC14L4 (SEC14L4 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73233-plasmid-map-sequence-id-137211

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  15. STARD13A

    Plasmid
    #42384
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STARD13A (STARD13 Human, STARD13A)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. EYFP-Annexin-12

    Plasmid
    #56583
    Depositor
    Michael Davidson
    Insert
    Annexin (A4) (ANXA4 Human)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56583-plasmid-map-sequence-id-241190

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  17. ECFP-Annexin-12

    Plasmid
    #55340
    Depositor
    Michael Davidson
    Insert
    Annexin (A4) (ANXA4 Human)
    Use
    Tags
    ECFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55340-plasmid-map-sequence-id-335165

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  18. mCherry-Annexin-8

    Plasmid
    #54978
    Depositor
    Michael Davidson
    Insert
    Annexin (A4) (ANXA4 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. mTurquoise-CENPA-C-22

    Plasmid
    #55542
    Purpose
    Localization: Nucleus, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    CENPA (CENPA Human)
    Use
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. mWasabi-Annexin-12

    Plasmid
    #56494
    Depositor
    Michael Davidson
    Insert
    Annexin (A4) (ANXA4 Human)
    Use
    Tags
    mWasabi
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. mTagRFP-T2-Annexin-12

    Plasmid
    #58034
    Depositor
    Michael Davidson
    Insert
    Annexin (A4) (ANXA4 Human)
    Use
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. mTagRFP-T-Annexin-12

    Plasmid
    #58002
    Depositor
    Michael Davidson
    Insert
    Annexin (A4) (ANXA4 Human)
    Use
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. mKOet-Annexin-12

    Plasmid
    #57910
    Depositor
    Michael Davidson
    Insert
    Annexin (A4) (ANXA4 Human)
    Use
    Tags
    mKOet
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. mKO-Annexin-12

    Plasmid
    #57832
    Depositor
    Michael Davidson
    Insert
    Annexin (A4) (ANXA4 Human)
    Use
    Tags
    mKO
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. mEmerald-Annexin-12

    Plasmid
    #53991
    Purpose
    Localization: Plasma Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Annexin (ANXA4 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pHH0103 KIAA1688 WW domain #1

    Plasmid
    #104277
    Purpose
    Bacterial expression of WW domain #1 from KIAA1688
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    KIAA1688 WW domain #1 (ARHGAP39 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104277-plasmid-map-sequence-id-202577

    Enlarge

  27. pHH0103 KIAA1688 WW domain #2

    Plasmid
    #104278
    Purpose
    Bacterial expression of WW domain #2 from KIAA1688
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    KIAA1688 WW domain #2 (ARHGAP39 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104278-plasmid-map-sequence-id-203477

    Enlarge

  28. mPlum-Annexin-12

    Plasmid
    #55951
    Depositor
    Michael Davidson
    Insert
    Annexin A4 (ANXA4 Human)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. mRuby-Annexin (A4)-8

    Plasmid
    #55846
    Purpose
    Localization: Plasma Membrane, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Annexin (A4) (ANXA4 Human)
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pET-24a-CXCR4

    Plasmid
    #133431
    Purpose
    Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133431-plasmid-map-sequence-id-267724

    Enlarge

  32. GIMAP1 (3LXW)

    Plasmid
    #25336
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GIMAP1:E25-R253 (GIMAP1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids E25-R253
    Promoter
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. rEstus

    Plasmid
    #209767
    Purpose
    To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.
    Depositor
    Stefan Heinemann
    Article
    Rühl et al bioRxiv 2023.
    Insert
    rEstus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209767-plasmid-map-sequence-id-413323

    Enlarge

  34. pLKO-Tet-On-ARNTL-shRNA2

    Plasmid
    #166914
    Purpose
    Lentivirus for inducible knockdown of human ARNTL(BMAL1)
    Depositor
    Roland Friedel
    Insert
    ARNTL shRNA (BMAL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166914-plasmid-map-sequence-id-331230

    Enlarge

  35. pLKO-Tet-On-ARNTL-shRNA1

    Plasmid
    #166913
    Purpose
    Lentivirus for inducible knockdown of human ARNTL(BMAL1)
    Depositor
    Roland Friedel
    Insert
    ARNTL shRNA (BMAL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166913-plasmid-map-sequence-id-425230

    Enlarge

  36. pLKO-Tet-On-ARNTL-shRNA3

    Plasmid
    #166915
    Purpose
    Lentivirus for inducible knockdown of human ARNTL(BMAL1)
    Depositor
    Roland Friedel
    Insert
    ARNTL shRNA (BMAL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166915-plasmid-map-sequence-id-331234

    Enlarge

  37. L57V

    Plasmid
    #34556
    Depositor
    Kevin Truong
    Insert
    L57 (CASP7 Human, A.Sativa (LOV2))
    Use
    Tags
    YFP
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic domain of caspase-7 was truncated at am…
    Promoter
    CMV, T7
    Available since
    April 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  38. L57R

    Plasmid
    #34557
    Depositor
    Kevin Truong
    Insert
    L57 (CASP7 Human, A.Sativa (LOV2))
    Use
    Tags
    mRFP
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic domain of caspase-7 was truncated at am…
    Promoter
    CMV, T7
    Available since
    April 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  40. mEos4b-Annexin-12

    Plasmid
    #57501
    Purpose
    Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    Annexin (ANXA4 Human)
    Use
    Tags
    mEos4b
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57501-plasmid-map-sequence-id-210780

    Enlarge

  49. RICS

    Plasmid
    #25208
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RICS:E18-F218 (ARHGAP32 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 301 - 350 of 613 results