We narrowed to 1,504 results for: asl

Showing: 301 - 350 of 1504 results

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pEF-pIgH-ESCargo(FTV)

Plasmid

#140160

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pESC-URA::CYP71AV1/CPR

Plasmid

#20118

Depositor

Jay Keasling

Article

Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

Insert

amorphadiene oxidase and p450 reductase

Use

Synthetic Biology

Expression

Yeast

Available Since

March 2, 2009

Availability

Academic Institutions and Nonprofits only
pCI-HA-ERK5-FL

Plasmid

#31817

Depositor

Astar Winoto

Article

Kasler et al Mol Cell Biol. 2000 Nov;20(22):8382-9.

Insert

Erk5 (Mapk7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140168

Purpose

Expresses a regulatable secretory cargo for Tetrahymena thermophila

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Other

Promoter

MTT1

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo*

Plasmid

#140159

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-DsRed-Express2-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Orange)

Plasmid

#140161

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-HaloTagx2

Plasmid

#177113

Purpose

Expresses Vps8-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

VPS8 (VPS8 Budding Yeast)

Expression

Yeast

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pconst #5-RFP

Plasmid

#81079

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23110

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #1-RFP

Plasmid

#81075

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23119

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #4-RFP

Plasmid

#81078

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23107

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSB74

Plasmid

#19591

Depositor

Jay Keasling

Article

Bang et al Appl Environ Microbiol. 2000 Sep . 66(9)

Insert

thiosulfate reductase (phsB S. enterica serovar Typhimurium LT2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
pUASt-ssBiP-ESCargo*

Plasmid

#140165

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-DsRed-Express2-FKBP(LV)(C22V)

Expression

Insect

Promoter

hs70

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pconst #3-RFP

Plasmid

#81077

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23101

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter- BBa_J23104

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSY091

Plasmid

#110068

Purpose

Bacterial expression of L4

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L4

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSY075

Plasmid

#110067

Purpose

Bacterial expression of L2

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L2

Expression

Bacterial

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSB77

Plasmid

#19590

Depositor

Jay Keasling

Article

Bang et al Appl Environ Microbiol. 2000 Sep . 66(9)

Insert

thiosulfate reductase

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
pUCHR-mClover-MT-C34

Plasmid

#177153

Purpose

Lentiviral vector for expression of anti-HIV-1 MT-C34 peptide on cell surface

Depositor

Dmitriy Mazurov

Article

Maslennikova et al mBio. 2022 Jan 25:e0358921. doi: 10.1128

Insert

mClover-MT-C34

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140170

Purpose

Expresses a regulatable secretory cargo for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140169

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo(Orange)

Plasmid

#140157

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Orange)

Plasmid

#140158

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Crimson)

Plasmid

#140156

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Plasmid

#140155

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#115428

Purpose

Expresses a non-glycosylated regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR I206F (JBEI-3678)

Plasmid

#110149

Purpose

SaGGR site-directed mutation of I206F

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of I206F

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR G91H (JBEI-3677)

Plasmid

#110148

Purpose

SaGGR site-directed mutation of G91H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of G91H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR L377H (JBEI-3683)

Plasmid

#110150

Purpose

SaGGR site-directed mutation of L377H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of L377H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
const #7-RFP

Plasmid

#81081

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23109

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pconst #6-RFP

Plasmid

#81080

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23117

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2olig-mCherry

Plasmid

#60032

Purpose

Expresses a fusion of CRY2PHR E490G with mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2PHR E490G (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

E490G

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CIB81-pmGFP

Plasmid

#75366

Purpose

Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

prenylated EGFP

Expression

Mammalian

Mutation

residues 1-81 of CIB1

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CRY2olig(FL)-mCherry

Plasmid

#60033

Purpose

Expresses a fusion of full length CRY2 (E490G) with mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2 E490G (deltaNLS) (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

E490G, NLS mutated

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKB3-SaGGR-I206F-L377H (JBEI-7202)

Plasmid

#110152

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-I206F-L377H mutant gene

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJet-gR-X4ex2-DTS

Plasmid

#177154

Purpose

Donor plasmid for knock-in of MT-C34 peptide into exon-2 of CXCR4

Depositor

Dmitriy Mazurov

Article

Maslennikova et al mBio. 2022 Jan 25:e0358921. doi: 10.1128

Insert

MT-C34-pA with CXCR4 arms of homology

Use

Pcr cloning

Promoter

none

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKB3-SaGGR-F219L (JBEI-7186)

Plasmid

#110151

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-F219L mutant gene

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2(535, L348F)-mCherry

Plasmid

#75372

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-535 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19-TdTwt

Plasmid

#207466

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTwt (Dntt Mouse)

Tags

10xHis

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Expression

Mammalian

Promoter

EF-1a

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2(L348F)-CreN

Plasmid

#75368

Purpose

Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Cre recombinase, residues 19-104

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2PHR(W349R)-mCherry

Plasmid

#75370

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-498 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

W349R

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4BD-CRY2(535)

Plasmid

#75369

Purpose

Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-ManII-GFP

Plasmid

#160905

Purpose

Expresses the Golgi marker ManII-GFP in mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

MAN2A1 (MAN2A1 Human)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellulose

Plasmid

#36300

Depositor

Jay Keasling

Article

Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

Insert

pCellulose

Use

Synthetic Biology

Promoter

Two promoters from the E coli genome drive two di…

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS18

Plasmid

#24629

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

mFar1 (Far1 Mouse)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
JBEI-3716

Plasmid

#100169

Purpose

Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

Depositor

Jay Keasling

Article

Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

Insert

codon-optimized MK

Expression

Bacterial

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 1,504 results for: asl