We narrowed to 11,272 results for: 110

1. pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

   Plasmid

   #110558

   Purpose

   miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   lacIq-Ptac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq-Ptac

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 Paf1

   Plasmid

   #11059

   Depositor

   Matthew Meyerson

   Article

   Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

   Insert

   Paf1 (PAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1D V5-His-TOPO-HuR.wt

   Plasmid

   #110417

   Purpose

   Mammalian expression of HuR (wild type)

   Depositor

   Sandra Martha Gomes Dias

   Insert

   HuR (ELAVL1) (ELAVL1 Human)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human USP30 (64-502, construct 8, MG-26-21)

   Plasmid

   #110745

   Purpose

   Bacterial expression of human USP30 (construct 8)

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Use

   Tags

   N-His6-3C-GST-3C

   Expression

   Bacterial

   Mutation

   F348D, M350D, I353E + insertion deletion: 64-357;…

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACEC3GH (Hyg)

   Plasmid

   #110084

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmTurquoise2-Drp1

   Plasmid

   #110946

   Purpose

   Expresses dynamin-like protein 1 fused to mTurquoise2

   Depositor

   Pablo Rivera-Fuentes

   Insert

   Dynamin-1-like protein (DNM1L Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p178 Grg-1L Myc His

   Plasmid

   #11067

   Depositor

   Ramesh Shivdasani

   Article

   Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

   Insert

   Grg1-L (Tle1 Mouse)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. tet-pLKO.puro_shLuc

   Plasmid

   #110422

   Purpose

   shLuc control, silence Luc gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   Luciferase
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO (RNA PolIII)

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCRY1 HA tag

   Plasmid

   #110298

   Purpose

   full length wt mouse Cry1 HA tag in pcDNA3.1

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   wt mcry1 HA tag (Cry1 Mouse)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   wildtype

   Promoter

   CMV

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQC V5 MCS IRES Puro

    Plasmid

    #110342

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-CAGGS-dCas9

    Plasmid

    #110823

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. H52-pUCM-hNGN2-inv

    Plasmid

    #110492

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

    Depositor

    Michael Ward

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    

    Use

    CRISPR, Cre/Lox, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, EF-1 alpha, and TRE3G

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_SPF45

    Plasmid

    #110044

    Purpose

    Protein expression and purification of SPF45

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    SPF45 (RBM17 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3-Flag-SPRTN-E112A

    Plasmid

    #110215

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACT5FVMF(f)

    Plasmid

    #110549

    Purpose

    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

    Depositor

    David Russell

    Article

    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

    Insert

    EGFP, FV LTRs
    

    Use

    Qpcr control for titering foamy virus

    Tags

    Expression

    Mutation

    Promoter

    promoter: MLV LTR

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hy_pMT Flag-URH49 WT

    Plasmid

    #110127

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    April 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmTurquoise2-LC3

    Plasmid

    #110947

    Purpose

    Expresses the autophagosome marker LC3 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Article

    Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSKC11 - UTRQ

    Plasmid

    #110490

    Purpose

    Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

    Depositor

    Shankar Balasubramanian

    Article

    Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

    Insert

    NRAS 5'-UTR (bp 1-254) (NRAS Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. hCD4-mOrange

    Plasmid

    #110192

    Purpose

    Encodes for human CD4 coding sequence tagged with the mOrange FP

    Depositor

    Sergi Padilla Parra

    Article

    Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

    Insert

    hCD4 (CD4 Human)
    

    Use

    Tags

    mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SB_concave

    Plasmid

    #110102

    Purpose

    pBXPHM3 with scaffold Sybody of the concave library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the concave library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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