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PX459v2-ILK-gRNA 1-exon 1

Plasmid

#163320

Purpose

SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 1_Exon1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
PX459v2-ILK-gRNA 2-exon 8

Plasmid

#163321

Purpose

SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 2_Exon8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6F

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
ABE8e-Cas9NG

Plasmid

#203328

Purpose

Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

ABE8e-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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hSyn-GtACR2-ChRmine-tandem-eYFP

Plasmid

#192580

Purpose

AAV-vector for bidirectional optogenetic manipulation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

somBiPOLES

Use

AAV

Tags

EYFP- soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Octo_VirD2_NLS-Cas9-6xHis

Plasmid

#232296

Purpose

Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

Depositor

Hee-Sik Kim

Article

Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

Octo_VirD2_NLS-Cas9-6xHis

Use

CRISPR

Tags

6xHis and Octopine-type VirD2-derived NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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evoFERNY-Cas9NG

Plasmid

#203329

Purpose

Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

evoFERNY-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

Plasmid

#208039

Purpose

Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin

Depositor

James Sutherland, Rosa Barrio

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

PML (isoform IVa; 3MAS) (PML Human)

Use

Lentiviral

Tags

FLAG, N-terminal Split-TurboID

Expression

Mutation

3MAS mutant: K65R, K160R and K490R; mutated SIM

Promoter

EFS

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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DSPQ-HTT-TALEN2 BSD

Plasmid

#92246

Purpose

TALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NN

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

CAG

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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DSPQ-HTT-TALEN1 ZEO

Plasmid

#92245

Purpose

TALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HD

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

CAG

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHLsec-dasTMPRSS2

Plasmid

#207850

Purpose

Expresses directed activation strategy TMPRSS2 in mammalian cells, such as HEK293F- or CHO-cells

Depositor

Ingrid De Meester

Article

Verhulst et al J Extracell Vesicles. 2025 Mar;14(3):e70

Insert

transmembrane serine protease 2 (TMPRSS2 Human)

Use

Tags

AviTag, 8xHIS tag

Expression

Mammalian

Mutation

deleted amino acid 1-108, S251D R252D Q253D S254D…

Promoter

CAG

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCROPseq-VEX-optimized-scaffold

Plasmid

#203321

Purpose

Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR65Q

Plasmid

#92249

Purpose

HTT donor DNA with 65Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Tags

Expression

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR45Q

Plasmid

#92248

Purpose

HTT donor DNA with 45Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Tags

Expression

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR81Q

Plasmid

#92250

Purpose

HTT donor DNA with 81Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Tags

Expression

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR30Q

Plasmid

#92247

Purpose

HTT donor DNA with 30Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Tags

Expression

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pX335 HTT sgRNA-a

Plasmid

#87201

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTT

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

HTT (HTT Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pX335 HTT sgRNA-b

Plasmid

#87200

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting 5' UTR of HTT

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

HTT (HTT Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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LIC4B-His-TEV-TAK1-TAB1

Plasmid

#154874

Purpose

Insect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91

Depositor

Kenneth Westover

Article

Tan et al J Med Chem. 2015 Jan 8;58(1):183-96. doi

Insert

TAK1-TAB1 (TAB1 Human)

Use

Tags

LIC TEV His

Expression

Insect

Mutation

Codon optimized

Promoter

polyhedrin

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19_extendedTTE1564

Plasmid

#60999

Purpose

T7-transcription template for Tte mRNA used in SiM-KARTS

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

TTE1564 transcript

Use

Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Kristijan Ramadan, Ivana Marinovic-Terzic

Article

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Use

Tags

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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