We narrowed to 4,673 results for: pcr

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pMaCTag-P28

Plasmid

#120039

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCre

Plasmid

#236212

Purpose

Arabinose-inducible Cre recombinase

Depositor

Matthew Waldor

Publication

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

Cre recombinase

Expression

Bacterial

Promoter

araBAD promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCralbp-DsRed

Plasmid

#11158

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cralbp promoter (Rlbp1 Mouse)

Tags

DsRed2

Expression

Mammalian

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMaCTag-P13

Plasmid

#120024

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPRyl_AXP

Plasmid

#84608

Purpose

Introduces DSB at AXP locus in Y. lipolytica

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCRISPRyl_A08

Plasmid

#84610

Purpose

Introduces DSB at A08 locus in Y. lipolytica

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pKM154

Plasmid

#13036

Depositor

Kenan Murphy

Publication

Murphy et al Gene. 2000 Apr 4. 246(1-2):321-30.

Insert

cat, sacB

Expression

Bacterial

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagGFP2

Plasmid

#67166

Purpose

CRISPaint universal donor for TagGFP2 fusion tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TagGFP2

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P23

Plasmid

#120034

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMH3

Plasmid

#52528

Purpose

serves as PCR-template to generate HR donors for C-terminal GFP-tag

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP and Blasticigin resistance cassette

Use

Blunt cloning kit

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCR3.1-mIL2

Plasmid

#74467

Purpose

expression of mouse IL2 in mammalian cells

Depositor

Loree Heller

Publication

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL2 (Il2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRB17

Plasmid

#52527

Purpose

serves as template for PCR-mediated fusion of U6-promoter with sgRNA

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Drosophila U6C promoter

Use

CRISPR; Blunt cloning kit

Tags

T7-promoter

Expression

Insect

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pCR8 JARID2

Plasmid

#114443

Purpose

Entry vector for gateway cloning containing human JARID2 coding sequence

Depositor

Adrian Bracken

Publication

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

JARID2 (JARID2 Human)

Use

Gateway entry cloning

Promoter

None

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCR4-huNFATc1nuc

Plasmid

#23988

Depositor

Jerry Crabtree

Publication

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1 (NFATC1 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCR1307

Plasmid

#111133

Purpose

Expresses WDR48.

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

WDR48 (WDR48 Human)

Use

Lentiviral

Tags

HA

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_D17

Plasmid

#84611

Purpose

Introduces DSB at D17 locus in Y. lipolytica

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCRISPRyl_XPR2

Plasmid

#84609

Purpose

Introduces DSB at XPR2 locus in Y. lipolytica

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CaV2.1 (pCR3.1)

Plasmid

#26573

Depositor

Diane Lipscombe

Publication

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cacna1a (Cacna1a Mouse)

Expression

Mammalian

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles