We narrowed to 4,553 results for: pcr

1. pCRISPR3_BC4

   Plasmid

   #191859

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR3_BC3

   Plasmid

   #191858

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR3_BC1

   Plasmid

   #191856

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPR2_wt

   Plasmid

   #149386

   Purpose

   P. aeruginosa PA14 CRISPR2 locus

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR2_-6D

   Plasmid

   #149387

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6bp deleted downstream of the IHFprox site

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR2_-5D

   Plasmid

   #149388

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   5bp deleted downstream of the IHFprox site

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR2_+5D

   Plasmid

   #149390

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   5bp inserted downstream of the IHFprox site

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR2_IHF_Opt

   Plasmid

   #149395

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   IHFprox site is optimized at key positions needed…

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPR2_IHF_Mut

   Plasmid

   #149396

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   IHFprox site is mutated at key positions needed f…

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR2_motif_scram

    Plasmid

    #149397

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Upstream motif recognized by Cas1-2/3 during spac…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR

    Plasmid

    #186276

    Purpose

    Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira shortened CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKPcR5

    Plasmid

    #149467

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, PcaU(AM) repressor, P3B5B promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    PcaU(AM)-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3B5B

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR2.1_HBB_A

    Plasmid

    #87848

    Purpose

    pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA

    Depositor

    Marina Kleanthous

    Article

    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

    Insert

    Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    HBB(IVSI-110) β-thalassaemia misplicing mutation …

    Promoter

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR8_TaNAM-A1

    Plasmid

    #124574

    Purpose

    TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-A1
    

    Use

    Topo gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR8_TtNAM-B1

    Plasmid

    #124575

    Purpose

    TtNAM-B1 CDS in the Gateway-compatible pCR8 vector.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-B1
    

    Use

    Topo gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCR1300

    Plasmid

    #111119

    Purpose

    Expresses USP1_+_62903144.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    USP1 gRNA (USP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR1324

    Plasmid

    #111120

    Purpose

    Expresses WDR48_+_39093886.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    WDR48 gRNA (WDR48 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR1323

    Plasmid

    #111125

    Purpose

    Expresses non-targeting_01224 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    Non-targeting gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCR1178

    Plasmid

    #111104

    Purpose

    Expresses FANCF_-_22647294.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCF gRNA (FANCF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR1224

    Plasmid

    #111105

    Purpose

    Expresses FANCF_-_22647359.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCF gRNA (FANCF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge