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Addgene
Showing: 121 - 140 of 216 results

  3. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Availability
    Industry, Academic Institutions, and Nonprofits
    177334-plasmid-map-sequence-id-348602

    Enlarge

  4. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid
    #177335
    Purpose
    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Availability
    Industry, Academic Institutions, and Nonprofits
    177335-plasmid-map-sequence-id-348601

    Enlarge

  5. pBS mTSP-5

    Plasmid
    #12996
    Depositor
    Jack Lawler
    Insert
    TSP-5 (Comp Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  6. pDG04135

    Plasmid
    #196013
    Purpose
    Nanobody Zasp-Nano65 production
    Depositor
    Dirk Görlich
    Insert
    H14-SUMO-Cys- ZaspNano65-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    196013-plasmid-map-sequence-id-385939

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  7. pDG04136

    Plasmid
    #196014
    Purpose
    Nanobody Zasp-Nano66 production
    Depositor
    Dirk Görlich
    Insert
    H14-SUMO-Cys- ZaspNano66-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    196014-plasmid-map-sequence-id-385937

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  8. GabaARD-GFP/pCDNA

    Plasmid
    #197321
    Purpose
    Plasmid expressing an antigen targeting the GABA-A Receptor delta
    Depositor
    Stephen Moss
    Insert
    GABA A delta (GABRD Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197321-plasmid-map-sequence-id-392893

    Enlarge

  9. pHH0103 NEDD4L WW domain #1

    Plasmid
    #104204
    Purpose
    Bacterial expression of WW domain #1 from NEDD4L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4L WW domain #1 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104204-plasmid-map-sequence-id-203479

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  10. pHH0103 NEDD4L WW domain #2

    Plasmid
    #104205
    Purpose
    Bacterial expression of WW domain #2 from NEDD4L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4L WW domain #2 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104205-plasmid-map-sequence-id-203673

    Enlarge

  11. pHH0103 NEDD4L WW domain #3

    Plasmid
    #104206
    Purpose
    Bacterial expression of WW domain #3 from NEDD4L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4L WW domain #3 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104206-plasmid-map-sequence-id-202575

    Enlarge

  12. pHH0103 NEDD4L WW domain #4

    Plasmid
    #104207
    Purpose
    Bacterial expression of WW domain #4 from NEDD4L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4L WW domain #4 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104207-plasmid-map-sequence-id-202579

    Enlarge

  13. NEDD4L

    Plasmid
    #25169
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    nedd4l.0574.0947 (NEDD4L Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Hect Domain
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  14. 6DXH (USP05)

    Plasmid
    #119968
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    USP05 (USP5 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 171-290
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    119968-plasmid-map-sequence-id-232692

    Enlarge

  16. pGBW-m4133388

    Plasmid
    #152928
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    152928-plasmid-map-sequence-id-295940

    Enlarge

  17. pGBW-m4046647

    Plasmid
    #153940
    Purpose
    Yeast expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Availability
    Industry, Academic Institutions, and Nonprofits
    153940-plasmid-map-sequence-id-299997

    Enlarge

  18. pGBW-m4133410

    Plasmid
    #152852
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    152852-plasmid-map-sequence-id-295864

    Enlarge

  19. pGBW-m4133870

    Plasmid
    #152903
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;TapTag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    152903-plasmid-map-sequence-id-295915

    Enlarge

  20. pGBW-m4133721

    Plasmid
    #152946
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    152946-plasmid-map-sequence-id-310881

    Enlarge

Showing: 121 - 140 of 216 results