We narrowed to 215 results for: stem loop

1. pFH2.19_mCherry-MS2x2

   Plasmid

   #205534

   Purpose

   Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NLS-tdPCP-GFP

   Plasmid

   #183934

   Purpose

   PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdPCP-GFP
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFH2.20_mCherry-MS2x4

   Plasmid

   #205535

   Purpose

   Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDZ617 pKAN 12xPP7 V4

   Plasmid

   #72237

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   12xPP7 V4
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFH2.21_mCherry-MS2x6

   Plasmid

   #205536

   Purpose

   Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEFS-ccdB-12X MS2_mPGK-Puro

   Plasmid

   #177809

   Purpose

   Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

   Type

   Empty backbone

   Use

   Tags

   12X MS2 stem loops

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

   Plasmid

   #72240

   Purpose

   Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   GLT1 GAL1p 24xPP7 V3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA4TO_24xGCN4_PCC_24xPP7

   Plasmid

   #236094

   Purpose

   Constitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   24xGCN4-PCC-24xPP7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA4TO_24xGCN4_MUT_24xPP7

   Plasmid

   #236093

   Purpose

   Constitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   24xGCN4-MUT-24xPP7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSX2739

    Plasmid

    #200765

    Purpose

    Expresses mKate2-tag fused CDC-42 with 8xMS2(V1) stem loops in C. elegans, and the tissue-specific promoter col-19 is used.

    Depositor

    Suhong Xu

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    8xMS2
    

    Use

    Tags

    mKate2

    Expression

    Worm

    Mutation

    Promoter

    col-19

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFH3.3_Cre-MS2x12

    Plasmid

    #205559

    Purpose

    Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NLS-tdPCP-GFP-VPR

    Plasmid

    #183936

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP-VPR
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCI-MS2V5-PABPC1 MLLEmut

    Plasmid

    #65808

    Purpose

    expression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PABPC1 (PABPC2 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    MLLEmut

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPotef-cdc3CB16-NatR

    Plasmid

    #86465

    Purpose

    Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

    Depositor

    Michael Feldbrügge

    Article

    Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

    Insert

    Cdc3
    

    Use

    Ustilago maydis expression

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Potef

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDZ645 pKAN 1x-mCherry-12xPP7 V4

    Plasmid

    #73173

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    1x-mCherry
    

    Use

    Tags

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6_T7_INT[6xminimal_Puf1]

    Plasmid

    #107608

    Purpose

    Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    6x minimal Puf1 motif
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SLIRP.HMG6.wt.fl

    Plasmid

    #49821

    Purpose

    expression vector for MBP-SLIRP fusion

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    SLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
    

    Use

    Tags

    E. coli Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ATF4 19: (SL)RLuc.5’ATF4.GFP

    Plasmid

    #21865

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Stem Loop upstream of ATF4

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

19. ATF4 9: (SL+)5’ATF4.GFP

    Plasmid

    #21856

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Stem loop introduced at the 5' end of the mR…

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge