We narrowed to 223 results for: stem loop
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pEFS-ccdB-12X MS2_mPGK-Puro
Plasmid#177809PurposeAssembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'DepositorTypeEmpty backboneTags12X MS2 stem loopsExpressionMammalianPromoterEFSAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH2.214_mCherry-PP7x12
Plasmid#205556PurposeExpress mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer)DepositorInsertmCherry
ExpressionMammalianMutationWTPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.19_mCherry-MS2x2
Plasmid#205534PurposeExpress mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
ExpressionMammalianMutationWTPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP
Plasmid#183934PurposePCP tandem dimer binding to PP7 RNA stem loops; labeled with GFPDepositorInserttdPCP-GFP
TagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH2.20_mCherry-MS2x4
Plasmid#205535PurposeExpress mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
ExpressionMammalianMutationWTPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDZ617 pKAN 12xPP7 V4
Plasmid#72237PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert12xPP7 V4
UseCre/LoxExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH2.21_mCherry-MS2x6
Plasmid#205536PurposeExpress mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
ExpressionMammalianMutationWTPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
Plasmid#72240PurposeHistidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)DepositorInsertGLT1 GAL1p 24xPP7 V3
ExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO_24xGCN4_PCC_24xPP7
Plasmid#236094PurposeConstitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.DepositorInsert24xGCN4-PCC-24xPP7
ExpressionMammalianAvailable SinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO_24xGCN4_MUT_24xPP7
Plasmid#236093PurposeConstitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.DepositorInsert24xGCN4-MUT-24xPP7
ExpressionMammalianAvailable SinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSX2739
Plasmid#200765PurposeExpresses mKate2-tag fused CDC-42 with 8xMS2(V1) stem loops in C. elegans, and the tissue-specific promoter col-19 is used.DepositorInsert8xMS2
TagsmKate2ExpressionWormPromotercol-19Available SinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH3.3_Cre-MS2x12
Plasmid#205559PurposeExpress Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertCre
ExpressionMammalianMutationWTPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VPR
Plasmid#183936PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VPR
TagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-PABPC1 MLLEmut
Plasmid#65808Purposeexpression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.DepositorInsertPABPC1 (PABPC2 Human)
TagsMS2 stem loops and V5ExpressionMammalianMutationMLLEmutPromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPotef-cdc3CB16-NatR
Plasmid#86465PurposeExpresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.DepositorInsertCdc3
UseUstilago maydis expressionTagsmCherryExpressionBacterialPromoterPotefAvailable SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDZ645 pKAN 1x-mCherry-12xPP7 V4
Plasmid#73173PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert1x-mCherry
ExpressionMammalian and YeastAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6_T7_INT[6xminimal_Puf1]
Plasmid#107608PurposeTransient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoterDepositorInsert6x minimal Puf1 motif
UseSynthetic BiologyExpressionMammalianPromoterU6Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLIRP.HMG6.wt.fl
Plasmid#49821Purposeexpression vector for MBP-SLIRP fusionDepositorInsertSLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
TagsE. coli Maltose Binding ProteinExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATF4 19: (SL)RLuc.5’ATF4.GFP
Plasmid#21865DepositorAvailable SinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only
