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Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

Plasmid

#111398

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

Plasmid

#111399

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

Plasmid

#111400

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

Plasmid

#111451

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160C

Plasmid

#46

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

expresses aa580-1330

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(1-200)

Plasmid

#43

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(400-580)

Plasmid

#45

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

expresses only aa400-580

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(200-400)

Plasmid

#44

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Expresses only aa200-400

Promoter

Available since

July 21, 2005

Availability

Academic Institutions and Nonprofits only
TFORF1269

Plasmid

#143482

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HIVEP2 (HIVEP2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRM053 His-TEV-Cas9 triNLS 0C

Plasmid

#196244

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TH0901-pOCC177-FUS-wt_opt(Nhe)

Plasmid

#221885

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRM049 His-TEV-Cas9 triNLS 2C

Plasmid

#196245

Purpose

Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TH0994-pOCC177-Fus-wt_(1-211)

Plasmid

#221886

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

PLD domain (aa 1-211)

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH1148-pOCC177-FUS(Nhe)-PLD_YtoF

Plasmid

#221887

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

In PLD all Tyr (Y) changed to Phe (F)

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH1149-pOCC177-FUS(Nhe)-RGG_RtoK

Plasmid

#221888

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

In RGG (aa 212-526) most of Arg (R) changed to Ly…

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0751

Plasmid

#143729

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HIVEP1 (HIVEP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1ΔN (MT2B2)-MutTER

Plasmid

#178035

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

First N-Terminal 27aa are deleted and Changed T(8…

Promoter

T7LacI

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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4GLM

Plasmid

#51313

Depositor

Cheryl Arrowsmith

Insert

4GLM (DNMBP Human, 4GLM)

Use

Tags

His

Expression

Bacterial

Mutation

Truncation: See Comments

Promoter

T7

Available since

Feb. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pEGFP‐RNASEH2A

Plasmid

#108700

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2A (RNASEH2A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2B

Plasmid

#108697

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2B (RNASEH2B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge