Addgene

We narrowed to 6,348 results for: Mag

Showing: 2071 - 2100 of 6348 results

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pC4EN-F1-mGAF670 (FKBP-mGAF670)

Plasmid

#80003

Purpose

miRFP670 GAF domain fused to FKBP

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FKBP

Use

Tags

GAF domain of miRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pABY-c25

Plasmid

#201769

Purpose

Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Use

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Mutation

Promoter

CYC1

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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ATM N-terminal sgRNA

Plasmid

#207089

Purpose

pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATCATTAAGTACTAGACTCA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAG463-FKBP-redNFAST

Plasmid

#160364

Purpose

Expresses FKBP-redNFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

FKBP-redNFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pABY-c15

Plasmid

#201768

Purpose

Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Use

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-lexAp65

Plasmid

#138393

Purpose

RMCE vector for generating C-terminal T2A-lexA-p65 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-lexAp65

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6-mTFP1-KanMX6

Plasmid

#69851

Purpose

C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBRHDAd-TKVenus

Plasmid

#26422

Depositor

Kohnosuke Mitani

Article

Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

HSV-TK, CS2-Venus

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pIM700

Plasmid

#74639

Purpose

alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pWA PAS-E BAD K-GAFm

Plasmid

#39866

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Inserts

iRFP protein PAS domain
iRFP protein GAF domain (mutated)

Use

Tags

E coil and K coil

Expression

Bacterial

Mutation

F165Y, D232Y, W308R

Promoter

arabinose inducible and rhamnose inducible

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO OM-eLIR

Plasmid

#189004

Purpose

mitophagy-inducing adaptor protein

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

OM-eLIR

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.16_MiniGagZip-MCP_Zip-P2A-eGFP

Plasmid

#205531

Purpose

Express HIV MiniGagZip-MCP_Zip-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

MiniGagZip-MCP_Zip

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_iRFP670

Plasmid

#191749

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and iRFP670

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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GeNL(Ca2+)_480/pcDNA3

Plasmid

#85205

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_480

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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Kohinoor 2.0/pcDNA3

Plasmid

#182315

Purpose

Positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Wazawa et al Microscopy (Oxf). 2021 Aug 9;70(4):340-3

Insert

Kohinoor 2.0

Use

Tags

No tag

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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CytERM-dTomato

Plasmid

#98834

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Use

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP713

Plasmid

#45465

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Use

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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Lyn-OeNL_pcDNA3

Plasmid

#89528

Purpose

Orange color variant of bright luminescent protein, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

Lyn

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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iRFP-PAmCherry1-PH_GRP1

Plasmid

#92168

Purpose

PI(3,4,5)P3 single molecule fluorescent reporter

Depositor

Xuelin Lou

Article

Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

Insert

iRFP-PAmCherry1-PHgrp1

Use

Tags

iRFP-PAmCherry1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Cyto-GCaMP3

Plasmid

#44331

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Insert

GCaMP3

Use

AAV; Adeno-associated virus ()

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BREP

Plasmid

#172340

Purpose

AAV transfer plasmid for BREP (bioluminescent red protein)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pVimentin-LSSmOrange

Plasmid

#37134

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human cytochrome C oxidase subunit VIII and human…

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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MXS_tdTomato

Plasmid

#62407

Purpose

MXS_chaining vector with tdTomato

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

tdTomato

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGKO2-Gateway

Plasmid

#63618

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants. Uses Gateway cloning.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Tags

Expression

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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BLING 1

Plasmid

#171647

Purpose

Expresses bioluminescent glutamate indicator in mammalian cells

Depositor

Assaf Gilad

Article

Petersen et al ACS Synth Biol. 2023 Jul 14. doi: 10.102

Insert

BLING 1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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tubulin-ReNL_pcDNA3

Plasmid

#89530

Purpose

Red color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAG375-H2B-redFAST

Plasmid

#160360

Purpose

Expresses H2B-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

H2B-redFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-Tubulin-6

Plasmid

#54291

Purpose

Localization: Microtubules, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Tubulin (TUBA1B Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
VV246: CoChR(C108S)-GCaMP6f-ER in fck

Plasmid

#58521

Purpose

Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

CoChR(C108S)-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 108 to Serine

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-WT

Plasmid

#30370

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN (PTEN Human)

Use

Tags

Expression

Mammalian

Mutation

wild type

Promoter

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only