We narrowed to 8,358 results for: BLI;

1. pAAVS1-CAG-hCAS9-neo

   Plasmid

   #166026

   Purpose

   AAVS1 targeting vector carrying CAG-SpCas9-neo

   Depositor

   Kosuke Yusa

   Insert

   AAVS1-CAGCas9IRESneo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBacMam7_N_Flag_TEV

   Plasmid

   #178817

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   Flag, TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-V2R

   Plasmid

   #37061

   Depositor

   Jurgen Wess

   Insert

   vasopressin 2 receptor (AVPR2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pJO672 (pRS415 Gal1-Ufd4-flag)

   Plasmid

   #159590

   Purpose

   expresses Ufd4-flag in yeast cells

   Depositor

   Alexander Varshavsky

   Insert

   Ufd4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIX403-iRFP-ccdB-Puro

   Plasmid

   #158566

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TMC1-TwinStrepII

   Plasmid

   #83267

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   TMC1 (Tmc1 Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTCF10 HLA-B*08:01

   Plasmid

   #180447

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-B*08:01
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti- V6.3 Ultra

   Plasmid

   #106172

   Purpose

   lentiviral CMV driven eGFP-P2A-T2A, Blasticidin selectable

   Depositor

   Ewa Snaar-Jagalska

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   T2A, P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_Efla_FLEX_APEX2-eGFPf

   Plasmid

   #171939

   Purpose

   Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein

   Depositor

   David Berson

   Insert

   APEX2-eGFPf
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-MBP-Geo_st

    Plasmid

    #87700

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Use

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE.is2-mOpn

    Plasmid

    #74411

    Purpose

    Expresses mouse Osteopontin from transcript tv2 in mammalian cells

    Depositor

    Georgios Stathopoulos

    Insert

    mouse Opn (Spp1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG-mito-dsRED2

    Plasmid

    #173071

    Purpose

    mitochondria targeted dsRED2 expressed off of CAG promoter

    Depositor

    Thomas Schwarz

    Insert

    Mito
    

    Use

    Lentiviral

    Tags

    dsRED

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB Neo* PB

    Plasmid

    #138413

    Purpose

    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

    Depositor

    Mario Capecchi

    Insert

    Neo*
    

    Use

    Mouse Targeting; Piggybac

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX4T3-Stx3(1-265)

    Plasmid

    #100573

    Purpose

    Expresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.

    Depositor

    Thomas Weimbs

    Insert

    Stx3-1-265 (Stx3 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    No transmembrane domain, residues 4-264 present.

    Promoter

    tac

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. GuideVector_BbsI

    Plasmid

    #110692

    Purpose

    For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.

    Depositor

    Douglas Mahoney

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPLICSFAST-ER-MT-P2A

    Plasmid

    #214892

    Purpose

    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEV-G2-Km ymScarletI

    Plasmid

    #193960

    Purpose

    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR_Hu_BIRC5(WT)

    Plasmid

    #136351

    Purpose

    Human BIRC5 cDNA in Gateway pENTR

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBacMam2_N_His8_eGFP_TEV

    Plasmid

    #178806

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    His8, eGFP, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTCF200 Mouse beta-2 microglobulin

    Plasmid

    #180462

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Mouse beta-2 microglobulin
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EGFP-Tubulin-6

    Plasmid

    #62736

    Purpose

    Localization: Microtubules, Excitation:488, Emission:507

    Depositor

    Michael Davidson

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pLIX403-EYFP-ccdB-Puro

    Plasmid

    #158549

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLIX403-EYFP-ccdB-Blast

    Plasmid

    #158551

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFRT-TODestFLAGHAhTTP

    Plasmid

    #48738

    Purpose

    Expresses hTTP

    Depositor

    Thomas Tuschl

    Insert

    TTP (ZFP36 Human)
    

    Use

    Tags

    FLAG, HA, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LentiGuide-10X-CS1-3'-puro

    Plasmid

    #212011

    Purpose

    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

    Depositor

    Danwei Huangfu

    Insert

    gRNA scaffold
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFastBacMam5_N_Flag_His8_eGFP_3C

    Plasmid

    #178816

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, His8, eGFP, 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ecALDH

    Plasmid

    #67921

    Purpose

    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Insert

    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFastBacMam6_C_3C_His8_Stop

    Plasmid

    #178810

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    3C, His8, Stop codon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid

    #218191

    Purpose

    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    NSP4 signal secretion peptide and myc

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLIX403-EBFP2-ccdB-Puro

    Plasmid

    #158552

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pExp-mCherry

    Plasmid

    #112569

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA5-FRT-TO-AID-EGFP

    Plasmid

    #80076

    Purpose

    Mammalian expression vector for C-terminal AID-EGFP tagging

    Depositor

    Andrew Holland

    Insert

    AID-EGFP
    

    Use

    Tags

    AID and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA5-FRT-TO-EGFP-AID

    Plasmid

    #80075

    Purpose

    Mammalian expression vector for N-terminal EGFP-AID tagging

    Depositor

    Andrew Holland

    Insert

    EGFP-AID
    

    Use

    Tags

    AID and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pT7-V5-SBP-C1-HshnRNPE1

    Plasmid

    #64921

    Purpose

    Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells

    Depositor

    Elisa Izaurralde

    Insert

    hnRNPE1 (PCBP1 Human)
    

    Use

    Tags

    V5 and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CROPSeq-PP7-p65-HSF

    Plasmid

    #196719

    Purpose

    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAGIG-6xHIS.Mm.RPE65-eGFP

    Plasmid

    #41019

    Depositor

    John Nickerson

    Insert

    RPE65 (Rpe65 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-ePEG-LINE1-loxPsym

    Plasmid

    #216866

    Purpose

    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

    Depositor

    Leopold Parts

    Insert

    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJJR81

    Plasmid

    #75029

    Purpose

    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Insert

    tagBFP2^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized tagBFP2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV-NT-3-mCherry

    Plasmid

    #190201

    Purpose

    To express the mouse NT-3 tagged with the FP mCherry

    Depositor

    Pantelis Tsoulfas

    Insert

    NT-3
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    The codon were optimized for better expression

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge