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Plasmid#191742PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mCherryExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCW008
Plasmid#83526Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha2
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTMS2
Plasmid#87800PurposeFor constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC572
Plasmid#62318PurposesgRNA with 1x com for yeast cellsDepositorInsertsgRNA + 1x com RNA binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTEF-Sup35C
Plasmid#1202DepositorInsertSup35 C-terminus (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationSup35 C-terminus ~254aa-endPromoterTEFAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFLIP37
Plasmid#65505PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-Cer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTNS1
Plasmid#87801PurposeFor constructing NusA-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYC44
Plasmid#63903PurposeInitial Integrative VectorDepositorTypeEmpty backboneUseTagsFRT::3'UTRcta::NAT::FRTExpressionYeastMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pR184
Plasmid#47952PurposertTA positive feedback loop with three tet operators in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterGal1Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-CDC12(WT)
Plasmid#1168DepositorInsertCDC12 (CDC12 Budding Yeast)
UseTagsGFPExpressionYeastMutationAdded N-terminal GFP to CDC12 in plasmid CDC12(WTā¦PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG424-ccdB
Plasmid#70124PurposeYeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterNoneAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCH001
Plasmid#47953PurposetetO-GFP reporter casetteDepositorInsertGFP
UseTet inducible expressionTagsExpressionYeastMutationPromoterT3Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F55
Plasmid#23079DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 9 to Arginine, H3 K9R ā¦PromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pVEG17*
Plasmid#111856PurposeXylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)DepositorInsertGAL1p-GAL3-Syn4.1-TEFt
UseSynthetic BiologyTagsExpressionYeastMutationD68N, V69M, A109V, I271LPromoterGAL1pAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-SEC7-Cerulean
Plasmid#18851DepositorInsertSEC7 (SEC7 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.clr-2.Y-SUP4t
Plasmid#89692PurposegRNA for clr-2 locusDepositorInsertgRNA for clr-2 locus
UseCRISPR; GrnaTagsNoExpressionMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
68Q-ymsfGFP
Plasmid#128560PurposeHttex1 (without proline-rich region) fused to ymsfGFPDepositorInsert68Q-Httex1 (without proline-rich region) fused to ymsfGFP
UseTagsymsfGFPExpressionYeastMutationPromoterGAL1Available sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMMRSF1-16
Plasmid#20177DepositorInsertsrc homology domain 3 (CRK Human)
UseTagsHis and NpuDnaEC16ExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCuHA-ATG13[3SA](426)
Plasmid#122076PurposeExpressing HA-ATG13[3SA]DepositorInsertATG13
UseTags3HAExpressionYeastMutationSerine 646 to alanine; Serine 649 to alanine; Serā¦PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
SepOTSγ
Plasmid#68284PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EF-Sep21, 1xtRNA-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC548
Plasmid#62316PurposesgRNA with 1x PP7 for yeast cellsDepositorInsertsgRNA + 1x PP7
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCH043
Plasmid#47949PurposertTA under the control of GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterGal1Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCH044
Plasmid#47950PurposertTA under the control of GAL2 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterGal2Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
SepOTSε
Plasmid#68286PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EF-Sep21, 1xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSĪø
Plasmid#68289PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 2xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/p82
Plasmid#1105DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationwild-type HSP82 regulated by the strong constitutā¦PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G170D
Plasmid#1113DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 170 changed to aspartic acid; ā¦PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHYRSF236
Plasmid#46377DepositorInsertNpuDnaE intein delta variant, inactive
UseTagsH6-Smt3ExpressionBacterialMutationC1A, C+1A, deleted T76, V97, D98, L100 from NpuDnā¦PromoterT7Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRC02
Plasmid#114681PurposesfGFP.Strand10-HaloenzymeDepositorInsertsfGFP.Strand10-Haloenzyme
UseTagsHis10ExpressionBacterialMutationPromoterT7Available sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Hsh155 K740R
Plasmid#111953PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740R mutationDepositorInsertHsh155 K740R
UseTagsExpressionBacterial and YeastMutationK740RPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-C67Y
Plasmid#10982DepositorInsertrpb1-C67Y (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 200 G to A in the RPB1 ORF resulting iā¦PromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-C70Y
Plasmid#10983DepositorInsertrpb1-C70Y (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 209 G to A in the RPB1 ORF resulting iā¦PromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-H80Y
Plasmid#10984DepositorInsertrpb1-H80Y (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 238 C to T in the RPB1 ORF resulting iā¦PromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-YPT52
Plasmid#18843DepositorInsertYPT52 (YPT52 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
sup35-C653R
Plasmid#1198DepositorInsertSup35p (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationchanged cys 653 to argPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-YPT1
Plasmid#18848DepositorInsertYPT1 (YPT1 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-YPT6
Plasmid#18845DepositorInsertYPT6 (YPT6 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJLI-Sup35?M
Plasmid#1225DepositorInsertSUP35 (SUP35 Budding Yeast)
UseYeast integrative plasmidTagsExpressionMutationdeletion of aa 124-253 of SUP35PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFLIP30
Plasmid#65499PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSν
Plasmid#68294PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EF-Sep21, 5xtRNA-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSβ
Plasmid#68283PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 1xtRNA-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
sup35-R320I
Plasmid#1199DepositorInsertSup35p (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationchanged arg 320 to isoleucinePromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFLIP32
Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-YPT31
Plasmid#18849DepositorInsertYPT31 (YPT31 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJD100
Plasmid#23085DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 4 to Glutamine, H3 K4Q.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJD101
Plasmid#23086DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 4 to Arginine, H3 K4R.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only