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CD11b promoter in pGEM3zf(-)

Plasmid

#26168

Depositor

Daniel Tenen

Article

Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

Insert

CD11b promoter (ITGAM Human)

Expression

Bacterial

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pNW55

Plasmid

#22988

Purpose

Expression of miRNA precursor for the osa-MIR528 locus in rice.

Depositor

Detlef Weigel

Article

Warthmann et al PLoS One. 2008 . 3(3):e1829.

Insert

osa-MIR528

Use

RNAi; Plant gene inactivation

Expression

Plant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

Plasmid

#141387

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro

Plasmid

#141393

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9c

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET-Sac-Abeta(M1-40)

Plasmid

#71876

Purpose

Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-40) (APP Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-EGFP-WPRE

Plasmid

#51502

Purpose

Can be used to generate AAV virus that will express EGFP in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV Retrograde, AAV2, AAV5, AAV8, and AAV9

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP delta

Plasmid

#12559

Depositor

Peter Johnson

Article

McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

Insert

CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)

Expression

Mammalian

Mutation

S2G introduced during cloning

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pSKI015

Plasmid

#11570

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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MSP2443

Plasmid

#72252

Purpose

Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-hSox2

Plasmid

#20073

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hSox2 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-SENP1m

Plasmid

#17358

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R630L, K631M

Available Since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB 54L

Plasmid

#19302

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T54L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

Plasmid

#100848

Purpose

AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCherry.90 alpha

Plasmid

#108222

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 alpha

Depositor

Didier Picard

Article

Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

Insert

mCherry-Hsp90a (HSP90AA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-LaG16-MNase

Plasmid

#170978

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

Insert

Glutathione-S-Transferase/LaG16/MNase

Tags

GST

Expression

Bacterial

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-tdTomato-WPRE

Plasmid

#51503

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

pCAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pSP6-RNAP

Plasmid

#48143

Purpose

Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

rna polymerase SP6

Use

Synthetic Biology

Expression

Bacterial

Mutation

V314A (numbering based on NCBI Sequence ID: NP_85…

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pPyCAG-Nanog-IP

Plasmid

#13838

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

None

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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