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Lenti_AID-Dam-lamin B1

Plasmid

#119764

Purpose

Lentiviral plasmid for Mammalian Expression

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

AID-Dam-lamin B1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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R26P-SA-EGFP-puro

Plasmid

#26890

Depositor

Rudolf Jaenisch

Article

Blelloch et al Proc Natl Acad Sci U S A. 2004 Sep 28. 1

Insert

EGFP

Use

Mouse Targeting

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

Plasmid

#192664

Purpose

3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al Cell Rep. 2026 Mar 24;45(3):117006. doi:

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, D839A, H840A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGATOR-DTA

Plasmid

#64103

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

Depositor

Bill Skarnes

Article

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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ACE2(-252)-luc

Plasmid

#31111

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -252 to +103

Available Since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD_GFP11_T7LysH17A

Plasmid

#59591

Purpose

Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

Depositor

Richard Kingston

Article

Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

Type

Empty backbone

Tags

6xHis-tag and GFP11

Expression

Bacterial

Promoter

pARA

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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EasyFusion mCherry

Plasmid

#112849

Purpose

General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mCherry

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PKCdelta-EGFP

Plasmid

#178856

Purpose

Mammalian expression of PKCδ fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ (PRKCD Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-2

Plasmid

#161819

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TRAIL-R1

Plasmid

#61382

Purpose

Expresses HA tagged TRAIL receptor 1

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 1

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R1 leader peptide (aa1-23) replaced with TN…

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) prME (JEV) sdm2

Plasmid

#52881

Purpose

expression of Japanese Encephalitis Virus VLPs in mammalian cells

Depositor

Yorgo Modis

Article

Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

Insert

prME (POLY Japanese encephalitis virus)

Expression

Mammalian

Mutation

sdm2

Promoter

CMV

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
PCS2+ Cas9

Plasmid

#122948

Purpose

For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cas9

Use

Synthetic Biology

Promoter

SP6

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRU45

Plasmid

#167667

Purpose

ENTRY vector for gRNA5 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn G51D

Plasmid

#105747

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

Insert

alpha-synuclein G51D (SNCA Human)

Expression

Bacterial

Mutation

G51D

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-ANP32A

Plasmid

#31563

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-neo.ARRDC1-mCherry

Plasmid

#38319

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP×3-N1

Plasmid

#86776

Purpose

A mammalian expression vector with triple EGFP at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTE105

Plasmid

#59400

Purpose

MethyloBrick expression vector, tuf promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptuf

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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RpH-ILV-3xALFA

Plasmid

#225135

Purpose

RpH-ILV probe strongly colocalises with lysosomal markers.

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

TMEM192 (TMEM192 Human)

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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