We narrowed to 16,532 results for: puromycin

1. pICE-EGFP-FLAG-TK1

   Plasmid

   #161919

   Purpose

   Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   Thymidine kinase, cytosolic (TK1 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-FLAG-2xSTREP-CCND2(T280A)-Puro

   Plasmid

   #172625

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr280Ala

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-FLAG-2xSTREP-CCND1-Puro

   Plasmid

   #172643

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. VEGF120TAG

   Plasmid

   #200946

   Purpose

   Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

   Depositor

   Elizabeth Kirby

   Article

   Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

   Inserts

   VEGF120TAG (Vegfa Mouse)
   hPGK-driven Puromycin resistance
   

   Use

   Lentiviral

   Tags

   6x HIS

   Mutation

   amber stop codon 101 bp from ATG

   Promoter

   Ef1a and hPGK

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. VEGF164TAG

   Plasmid

   #200947

   Purpose

   Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

   Depositor

   Elizabeth Kirby

   Article

   Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

   Inserts

   VEGF164TAG (Vegfa Mouse)
   hPGK-driven Puromycin resistance
   

   Use

   Lentiviral

   Tags

   6x HIS

   Mutation

   amber stop codon 101 bp from ATG

   Promoter

   Ef1a and hPGK

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-FLAG-2xSTREP-CCND1(P287A)-Puro

   Plasmid

   #172641

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(P287A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Ala

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-FLAG-2xSTREP-CCND1(T286A)-Puro

   Plasmid

   #172642

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr286Ala

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro

   Plasmid

   #172633

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr286Ala

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-2xFLAG-2xSTREP-CCND1-Puro

   Plasmid

   #172634

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-FLAG-2xSTREP-CCND3(P284L)-Puro

    Plasmid

    #172617

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(P284L) and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Pro284Leu

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-FLAG-2xSTREP-CCND2(P281L)-Puro

    Plasmid

    #172624

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Pro281Leu

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-PGK-flag-RAP1B-N17-puro

    Plasmid

    #156169

    Purpose

    Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-PGK-RAP2A-WT-puro

    Plasmid

    #156170

    Purpose

    Lentiviral vector for expression of RAP2A-WT, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-PGK-RAP2A-V12-puro

    Plasmid

    #156171

    Purpose

    Lentiviral vector for expression of RAP2A-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-PGK-RAP2A-N17-puro

    Plasmid

    #156172

    Purpose

    Lentiviral vector for expression of RAP2A-N17, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIX-hN1ICD

    Plasmid

    #91897

    Purpose

    Doxycycline inducible expression of human Notch1 ICD

    Depositor

    Julien Sage

    Insert

    Notch1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    codons 1770 to 2555 of human NOTCH1

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid

    #174901

    Purpose

    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG and NES

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EGFP-C1-centrin-1

    Plasmid

    #73331

    Purpose

    Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag

    Depositor

    Manuel Thery

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MEK1-DD-V5

    Plasmid

    #217298

    Purpose

    Lentivirus vector for expression of MEK1 S218D/S222D

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S218D/S222D

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX-EF1a_Empty

    Plasmid

    #221480

    Purpose

    Empty vector control for pLX307 overexpresion experiments

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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