We narrowed to 2,171 results for: D10

1. 3xFLAG-dCas9/pMXs-puro

   Plasmid

   #51240

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9 TadCBEe

   Plasmid

   #193836

   Purpose

   Express TadCBEe (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDe-SpCas9D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA031

   Plasmid

   #216011

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9-SpRY (D10A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 beta4

   Plasmid

   #16037

   Depositor

   Filippo Giancotti

   Article

   Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

   Insert

   beta4 (ITGB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA030

   Plasmid

   #216010

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9-SpG (D10A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_dCas9-2xAM

   Plasmid

   #92220

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA to be inserted into Bbs I sites
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CABE-T3.1

   Plasmid

   #193267

   Purpose

   Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T3.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AXBE

   Plasmid

   #204605

   Purpose

   Mammalian expression of adenine transversion base editor AXBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT130_GalL_nCDA1-NGBE3

   Plasmid

   #145094

   Purpose

   Expressing base editor nCDA1-NGBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-NGBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AA028

    Plasmid

    #216008

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9 (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AA029

    Plasmid

    #216009

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-NG (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IFNAR1_pCSdest

    Plasmid

    #53881

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. SpCas9 TadCBEa

    Plasmid

    #193782

    Purpose

    Express TadCBEa (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa-SpCas9D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NLS-dCas9-trCIB1

    Plasmid

    #64119

    Purpose

    Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    dCas9-trCIB1 fusion
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    dCas9 (D10A H840A, catalytically inactive), trCIB…

    Promoter

    CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. HUWE1-sgRNA-1

    Plasmid

    #86924

    Purpose

    To express sgRNA target HUWE1 gene

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    HUWE1 sgRNA1 (HUWE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

16. HUWE1-sgRNA-2

    Plasmid

    #86925

    Purpose

    To express sgRNA target HUWE1 gene

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    HUWE1 sgRNA2 (HUWE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

17. anti-FX(a) Emi HC

    Plasmid

    #113665

    Purpose

    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FIX(a) Emi HC and the Emi LC.

    Depositor

    Robert Peters

    Article

    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

    Insert

    Emicizumab anti-FX/FXa heavy chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJ609-dCas9-KR-puro

    Plasmid

    #158478

    Purpose

    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

    Depositor

    Brendan Price

    Article

    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

    Insert

    dCas9-KR
    

    Use

    CRISPR

    Tags

    KillerRed

    Expression

    Mammalian

    Mutation

    Cas9m4 (D10A+D839A+H840A+N863A)

    Promoter

    CMV

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCLXSN(GFP)-myc-BioID-hp100

    Plasmid

    #174738

    Purpose

    Gene overexpression

    Depositor

    Shao-Cong Sun

    Article

    Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

    Insert

    P100 (NFKB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

    Plasmid

    #162333

    Purpose

    Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSpCas9-2xVP64
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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