We narrowed to 3,037 results for: multiplex

1. pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115207

   Purpose

   Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   BzoCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115220

   Purpose

   Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   Pin3Cas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115214

   Purpose

   Expresses PauCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PauCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAC103-PBNeo-U6-sgRNA-Expression

   Plasmid

   #48228

   Purpose

   sgRNA expression on PiggyBac vector with Neo-selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCBC-DT2T3

   Plasmid

   #50591

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T2, gRNA scaffold, U6-29t plus, U6-1p, T3
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCBC-MT3T4

   Plasmid

   #50595

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-26t plus, OsU3p, T4
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEP163

   Plasmid

   #137881

   Purpose

   PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   mRFP-T2A-BlasticidinR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

   Plasmid

   #175526

   Purpose

   Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag11 and Tomm20-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL1119 (pDonor)

   Plasmid

   #160732

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. P1-EYFP-pA (Construct 5)

    Plasmid

    #55197

    Purpose

    Synthetic promoter responsive to gRNA1

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    EYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal Ade MLP

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-ExRai-AKAR

    Plasmid

    #118405

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. P2-ECFP-pA (Construct 6)

    Plasmid

    #55198

    Purpose

    Synthetic promoter responsive to gRNA2

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal Ade MLP

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-RAB-ICUE

    Plasmid

    #118480

    Purpose

    ddRFP-based single-color biosensor for monitoring cAMP dynamics.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-ICUE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro

    Plasmid

    #210604

    Purpose

    heat shock promoter - activated hyperdCas12a-based transcriptional activator

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    hyperdCas12a-miniVPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    truncated hsp70

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-blueAKAR

    Plasmid

    #118470

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTA-HUH

    Plasmid

    #221152

    Purpose

    HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Insert

    HisG-URA3-HisG
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 8xNFAT-ZsG-mCD8

    Plasmid

    #162747

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 2xP53_RE::NLuc

    Plasmid

    #124533

    Purpose

    p53 NLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    P53 NLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TB:7xE-Box:MiniP

    Plasmid

    #133880

    Purpose

    7 copies of the E-box motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies of the E-box motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLifeAct-miRFP670nano

    Plasmid

    #127434

    Purpose

    F-Actin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    LifeAct-miRFP670nano
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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