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Plasmid#215827PurposeExpress CBE6d V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6d V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD141B
Plasmid#218139PurposeLentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.DepositorInsertSD56-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFudio-Cpg15-IRES2-TdTomatoW
Plasmid#134309PurposeExpresses cpg15 and TdTomato upon coexpression with Cre recombinaseDepositorInsertsUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterUbCAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-F in pAR-9
Plasmid#166921PurposeAAV rep/cap plasmid. Capsid is mouse brain tropic AAV-FDepositorInsertREP/CAP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826-BB0323-mCherryBb
Plasmid#118244PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoterDepositorInsertBB0323-mCherry translational fusion under the control of the bb0826 promoter
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
LC201: pMVP/PB/Puro-DEST
Plasmid#121874PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD3
Plasmid#20185DepositorInsertNotch 3 Intracellular Domain (Notch3 Mouse)
UseTags3X FlagExpressionMammalianMutationContains murine Notch 3 Intracellular Domain (Met…PromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#178087PurposeHDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertmScarlet-I mTOR signaling indicator (TOSI)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-TSF-TEVcs-ULK1
Plasmid#171416PurposeFor the purifcation of human ULK1 complexDepositorInsertULK1 (ULK1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRedCm
Plasmid#176225PurposeLambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phageDepositorInsertLambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-2A-Cas9
Plasmid#170805PurposeAll-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptideDepositorInsertCas9
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLC133
Plasmid#198639PurposeExpression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.DepositorInsertdCas9
UseTags3x-FLAGExpressionBacterialMutationPromoterpTetAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Level 1 P1 OsActinP:Hygint:NosT
Plasmid#165423PurposeGoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassetteInsertOs Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
UseSynthetic Biology; L1 p1(r) golden gate (moclo) h…TagsExpressionMutationPromoterOs Actin promoter :: Hygromycin resistance gene (…Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTO149_RFP_CauNEO
Plasmid#177277PurposeEncodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.DepositorInsertRFP, CauNEO
UseTagsENO1-RFPExpressionBacterialMutationAll CUG Leucine codons were replaced with the syn…PromoterAgTEF2Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6a-V106W
Plasmid#215824PurposeExpress CBE6a-V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6a V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB115
Plasmid#68569PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mito-GFP-ER tethering
Plasmid#160509PurposeRescue mfn2 knockdown by tethering mitochondria and ER with GFPDepositorInsertmito-GFP-ER tethering
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LB601: pMVP/PB/eGFP-DEST
Plasmid#121870PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-ATBF1
Plasmid#40927DepositorInsertATBF1 (ZFHX3 Human)
UseTagsFLAGExpressionMammalianMutationSee depositor comments below.PromoterCMVAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4in
Plasmid#176232PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4out
Plasmid#176233PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1 reporter
Plasmid#197275PurposeEncodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYB2 reporter
Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB116
Plasmid#68570PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNA500
Plasmid#149576Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
Plasmid#60815PurposedCas9 with single cysteine residue (M1C) for site-specific labellingDepositorInsertdCas9 M1C D10A C80S H840A C574S
UseTagsHis10 and MBPExpressionBacterialMutationM1C D10A C80S H840A C574SPromoterAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk3a1
Plasmid#13758DepositorInsertJnk3a1 (MAPK10 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB215
Plasmid#68606PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only