We narrowed to 8,358 results for: BLI;

1. pAc5.1B-EGFP-DmRRP40

   Plasmid

   #65797

   Purpose

   expresses EGFP-tagged RRP40 in Drosophila cells

   Depositor

   Elisa Izaurralde

   Insert

   RRP40 (Rrp40 Fly)
   

   Use

   Drosophila expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Actin 5C

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

   Plasmid

   #48284

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTre-Tight-luciferase-GFP (467)

   Plasmid

   #65491

   Purpose

   negative control for expression assays

   Depositor

   Rory Johnson, Roderic Guigo

   Insert

   EGFP
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJM104: pTrc RFP on pCM66T

   Plasmid

   #74745

   Purpose

   pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)

   Depositor

   Mary Lidstrom

   Insert

   RFP (BioBrick part number BBa_E1010)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   pTrc

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET3a-His-DJ1

   Plasmid

   #51488

   Purpose

   Inducible expression of human His-DJ1 in E.coli

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   DJ1 (PARK7 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 31, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. mouse ptc probe M2-3

   Plasmid

   #58701

   Purpose

   makes mouse ptc probe for in situ hybridization

   Depositor

   Matthew Scott

   Insert

   ptc (Ptch1 Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Bacterial

   Mutation

   only EcoRI fragment (5' end)

   Promoter

   T7

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pKSB- 3xP3-mTurqSV40 GGGG AAGA

   Plasmid

   #174533

   Purpose

   3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes

   Depositor

   Eric Marois

   Insert

   3xP3-mTurquoise BsaI cassette
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   3xP3

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-iRFP713(V256C)-P2A-EGFP

   Plasmid

   #198071

   Purpose

   The AAV vector for iRFP713(V256C) and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP713(V256C)
   

   Use

   AAV

   Tags

   P2A-EGFP

   Expression

   Mutation

   V256C mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRIOBP-TwinStrepII

   Plasmid

   #83201

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   TRIOBP (Triobp Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLIX403-EBFP2-ccdB-G418

    Plasmid

    #158556

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pExp-PpiB

    Plasmid

    #112572

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-PpiB and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pnos-Fok1:dCas9-nos

    Plasmid

    #62210

    Purpose

    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

    Plasmid

    #48311

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. MIG-REST

    Plasmid

    #91898

    Purpose

    Expression of mouse REST

    Depositor

    Julien Sage

    Insert

    REST (Rest Mouse)
    

    Use

    Retroviral

    Tags

    ires-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKT-PDGFRa-IRES-Katushka

    Plasmid

    #133081

    Purpose

    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    PDGFRa (Pdgfra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Sperm whale Mb 64Y68F in pVP80K

    Plasmid

    #86956

    Purpose

    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

    Depositor

    John Olson

    Insert

    Mb 64Y68F
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    64Y68F

    Promoter

    T5

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid

    #191529

    Purpose

    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    H2BK_mNG_hSLBP(18-108)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mNeonGreen (mNG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB-CAG-PE2-NatMX

    Plasmid

    #158567

    Purpose

    Piggybac vector that allows for the generation of PE2-expressing stable cell lines

    Depositor

    Alejandro Chavez

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLIX403-EBFP2-ccdB-Blast

    Plasmid

    #158557

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLXIN2-RFP

    Plasmid

    #99205

    Purpose

    RFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    Red fluorescent protein
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Tags

    Expression

    Mammalian

    Mutation

    The dsRed cDNA was PCR amplified off AddGene plas…

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK615

    Plasmid

    #71699

    Purpose

    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGAL-flp(H305L)-NAT

    Plasmid

    #160380

    Purpose

    overexpression of Flp recombinase step-arrest mutant in yeast

    Depositor

    John McCusker

    Insert

    FLP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHH0103 NEDD4L WW domain #2

    Plasmid

    #104205

    Purpose

    Bacterial expression of WW domain #2 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #2 (NEDD4L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG416GAL-EMCV_3C

    Plasmid

    #201941

    Purpose

    Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    EMCV 3C protease (EMCVgp1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUA97

    Plasmid

    #69340

    Purpose

    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

    Depositor

    Oliver Hobert

    Insert

    ceBFP SEC 3xFlag
    

    Use

    Tags

    3xFlag and ceBFP (mTagBFP)

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJK633

    Plasmid

    #72397

    Purpose

    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDTA-5

    Plasmid

    #128845

    Purpose

    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103 NEDD4 WW domain #1

    Plasmid

    #104208

    Purpose

    Bacterial expression of WW domain #1 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #1 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid

    #86668

    Purpose

    for generation of BirA tagged strains using CRISPR tagging technology

    Depositor

    David Sibley

    Insert

    Linker-BirA-3xHA-HXGPRT 3'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGHE-PdNIP2-1

    Plasmid

    #108447

    Purpose

    PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.

    Depositor

    Richard Bélanger

    Insert

    PdNIP2-1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pT7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKSB- 3xP3-GFPsv40 GGGG AAGA

    Plasmid

    #174531

    Purpose

    3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-GFP BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    3xP3

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Preprotachykinin A

    Plasmid

    #130664

    Purpose

    Expresses human Preprotachykinin A in mammalian cells

    Depositor

    Michael Kressel

    Insert

    Preprotachykinin A (TAC1 Human)
    

    Use

    Tags

    Fusion Red

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pE-SUMO CRYGD

    Plasmid

    #80755

    Purpose

    Expression of human gamma D-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGD (CRYGD Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103 NEDD4 WW domain #4

    Plasmid

    #104211

    Purpose

    Bacterial expression of WW domain #4 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #4 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLinker-6xHA-HXGPRT-LoxP

    Plasmid

    #86552

    Purpose

    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma

    Depositor

    David Sibley

    Insert

    Linker-6xHA-HGPRT-LoxP
    

    Use

    Expression in toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PB CAG-sfGFP-iCre-ERT2

    Plasmid

    #137856

    Purpose

    CAG-sfGFP-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-iCre-ERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CbSBS-Flag/TRRAP(S722F)

    Plasmid

    #32105

    Depositor

    Yardena Samuels

    Insert

    Transformation/transcription domain-associated protein (S722F) (TRRAP Human)
    

    Use

    Tags

    Flag 1x

    Expression

    Mammalian

    Mutation

    Changes serine 722 to Phe

    Promoter

    CMV

    Available since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pB6-ROSA26-DTA-Capecchi-for

    Plasmid

    #125743

    Purpose

    B6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG426GAL-KUNV_NS2B-GS-NS3

    Plasmid

    #203506

    Purpose

    Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    KUNV NS2B-GS-NS3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge