Addgene

We narrowed to 4,250 results for: TIL;

Showing: 1401 - 1420 of 4250 results

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pFA6a-kanMX6-P81nmt1-mCFP

Plasmid

#105142

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu, Thomas Pollard

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mCFP

Use

Tags

81nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mCFP

Plasmid

#105139

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu, Thomas Pollard

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mCFP

Use

Tags

41nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAEF6

Plasmid

#136303

Purpose

pAEF5 plasmid with two gRNA inserted

Depositor

Gilles Fischer

Article

Agier et al Methods Mol Biol. 2021;2196:181-198. doi

Insert

double cassette coding for gRNA

Use

CRISPR; Template for pcr to insert double grna co…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGGD007

Plasmid

#48837

Purpose

Provides linker:nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

linker:nuclear localization signal

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBluescript_VEGFA-TS3_SpCas9

Plasmid

#107328

Purpose

U6 driven SpCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescript_VEGFA-TS3_NmCas9

Plasmid

#107329

Purpose

U6 driven NmCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pvTRAP

Plasmid

#50047

Purpose

expresses plasmodium vivax TRAP adhesion domains (25-283) in mammalian cells

Depositor

Timothy Springer

Article

Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

Insert

plasmodium vivax TRAP

Use

Tags

6Xhis tag

Expression

Mammalian

Mutation

S42Q, N91S, N128S, S180R

Promoter

cmv

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pSC-hXRCC1-PreE2

Plasmid

#52278

Purpose

Bacterial expression of human XRCC1 C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Use

Tags

Ubc9-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x3

Plasmid

#63603

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-VP16AD

Plasmid

#26268

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

VP16AD-ZIP+

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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YSBE 731 : pHYG_4xFLAG

Plasmid

#92090

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

4xFlag

Use

tagging proteins in cryptococcus neoforman

Tags

Expression

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x5

Plasmid

#63599

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pQCXIN X2/pTER shLUC (w347-1)

Plasmid

#17489

Purpose

Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase shRNA

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-81nmt1

Plasmid

#105164

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-81nmt1

Use

Tags

81nmt1

Expression

Bacterial and Yeast

Mutation

Promoter

81nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-41nmt1

Plasmid

#105163

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-41nmt1

Use

Tags

41nmt1

Expression

Bacterial and Yeast

Mutation

Promoter

41nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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LexAop2->Bxb1.STOP>myr::4xSNAPf

Plasmid

#87640

Purpose

For creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x3

Plasmid

#63597

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pQCXIP X2/pTER shLUC (w454-1)

Plasmid

#17491

Purpose

Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase shRNA

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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RCAS(A) cIhh (CT#432)

Plasmid

#13998

Depositor

Cliff Tabin

Article

Vortkamp et al Science. 1996 Aug 2. 273(5275):613-22.

Insert

Ihh (IHH Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pXN-FBLWLF-GAL4AD

Plasmid

#26263

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Gal4AD-Zip+ (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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