We narrowed to 9,222 results for: yeast expression

1. pBBK32 pHomL-TEF2p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

   Plasmid

   #179016

   Purpose

   Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBBK30 pHomL-RPL18Bp-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179014

   Purpose

   Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBBK29 pHomL-TEF2p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179013

   Purpose

   Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBBK27 pHomL-RPL18Bp-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

   Plasmid

   #179011

   Purpose

   Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS307

   Plasmid

   #51785

   Purpose

   an integrating (YIp) vector with a LYS2 selectable marker

   Depositor

   David Stillman

   Article

   Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS415-IVb

   Plasmid

   #31461

   Depositor

   Ana Kitazono

   Article

   Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

   Insert

   linker
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pRS413-IVa

   Plasmid

   #31458

   Depositor

   Ana Kitazono

   Article

   Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

   Insert

   linker
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pRS413-IVb

   Plasmid

   #31459

   Depositor

   Ana Kitazono

   Article

   Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

   Insert

   linker
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pRS316-IVb

   Plasmid

   #31457

   Depositor

   Ana Kitazono

   Article

   Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

   Insert

   linker
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pCEC-red

    Plasmid

    #196040

    Purpose

    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

    Depositor

    Paola Branduardi

    Article

    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Grna cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHYX137

    Plasmid

    #202814

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScPCK1

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHYX138

    Plasmid

    #202815

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScADH2

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAS9i

    Plasmid

    #141249

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAS9cd

    Plasmid

    #141252

    Purpose

    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAS9c

    Plasmid

    #141250

    Purpose

    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACT2-CAS9c

    Plasmid

    #51055

    Purpose

    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    Cas9c
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    yeast ADH1 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSSa26

    Plasmid

    #186784

    Purpose

    Expression of reverse transactivator (CgrtTA)

    Depositor

    Dominique Sanglard

    Article

    Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

    Insert

    CgrtTA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScPGK1p

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CUP1-∆ssCPY*-mGFP

    Plasmid

    #212197

    Purpose

    Fluorescent reporter for cytoplasmic aggregates

    Depositor

    Mark Leake, Chris MacDonald

    Insert

    Carboxypeptidase Y variant (PRC1 Budding Yeast)
    

    Use

    Tags

    mEGFP (monomeric version EGFP, A206K mutation)

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Promoter

    CUP1

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-mCherry-hphMX6

    Plasmid

    #105156

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

    Insert

    mCherry-hphMX6
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNTI661 pRPR1(TetO)-sgRNA

    Plasmid

    #139475

    Purpose

    Vector for tet-regulated sgRNA expression in yeast

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Insert

    single guide RNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pRPR1(TetO)

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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