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Plasmid#197503PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32ā¦PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
6His-MBP-TEV-huAsCpf1
Plasmid#90095PurposeBacterial expression plasmid for protein purificationDepositorInserthuAsCpf1
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV siteā¦ExpressionBacterialMutationPromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NRAS_p.Q61R
Plasmid#81652PurposeGateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertNRAS (NRAS Human)
UseGateway entry vectorTagsExpressionMutationQ61RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-PLXNB2-shRNA1
Plasmid#98399PurposeLentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible)DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIG-VP16 RARa [TJ#389]
Plasmid#16287DepositorInsertRAR alpha (RARA Human)
UseTagsVP16ExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
NTSR2-Tango
Plasmid#66458PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertNTSR2 (NTSR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[himar1C9]
Plasmid#213906PurposeMariner transposon with ATc-inducible himar1C9 transposaseDepositorInsertmariner transposon with cam resistance and ATc-inducible himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA(Cm)
Plasmid#48886PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYCipi
Plasmid#53279PurposeContains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHel3
Plasmid#102961PurposeE.coli/H.Pylori shuttle vectorDepositorTypeEmpty backboneUseShuttle vectorTagsExpressionMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only