165,652 results

1. Nme2Cas9_AAV

   Plasmid

   #119924

   Purpose

   All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   AAV

   Tags

   NLS-3xHA-NLS and NLS-NLS

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-DIO-CAG-hM4Di-P2A-dTomato

   Plasmid

   #202037

   Purpose

   AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Insert

   hM4Di
   

   Use

   AAV

   Tags

   dTomato

   Promoter

   CAG

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSC-NLS-EGFP

   Plasmid

   #221811

   Purpose

   Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)

   Depositor

   Baojin Ding

   Article

   Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

   Insert

   NLS-EGFP
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFVdelCME-mVenus

   Plasmid

   #179953

   Purpose

   pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   YFV-17D lacking C, prM, E proteins but with mVenus
   

   Use

   Transneuronal tracing

   Promoter

   SP6

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TPC2-G-GECO1.2

   Plasmid

   #207141

   Purpose

   Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPC2N2 (TPCN2 Human)
   

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pABE8e-NG-403Q-Nterm-AAV

   Plasmid

   #194651

   Purpose

   N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG N-term
   

   Use

   AAV

   Tags

   BPNLS and NpuN spilt intein

   Promoter

   TNNT2

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pABE8e-NG-403Q-Cterm-AAV

   Plasmid

   #194652

   Purpose

   C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG C-term
   

   Use

   AAV

   Tags

   BPNLS and NpuC split intein

   Promoter

   TNNT2

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.2-Cx43-HA

   Plasmid

   #49851

   Purpose

   Encodes human connexin 43 with a C-terminal HA epitope fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Contains several wobble mutations to confer resis…

   Promoter

   CMV

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. VRQR-ABEmax

    Plasmid

    #119811

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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