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pCMV Flag WT-PTEN

Plasmid

#22231

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Sep 9. ():.

Insert

PTEN (PTEN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-SHP2 CS

Plasmid

#8382

Depositor

Ben Neel

Insert

SHP2 (PTPN11 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C459S, mutation of essential catalytic cysteine

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
dead Sa Cas9

Plasmid

#138162

Purpose

For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

dSaCas9

Use

Tags

NLS

Expression

Mammalian

Mutation

D10A + N580A

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Ki67-mNeonGreen-IRESpuro2

Plasmid

#183737

Purpose

Expresses Ki67-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shSTING-Hsa

Plasmid

#128158

Purpose

Doxycyclin inducible shRNA knockdown of human STING gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shSTING Hsa (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 alpha-actinin 1

Plasmid

#11908

Depositor

Carol Otey

Article

Edlund et al Cell Motil Cytoskeleton. 2001 Mar . 48(3

Insert

alpha-actinin 1 (ACTN1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pMK293 (mAID-mCherry2-Hygro)

Plasmid

#72831

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Hygro

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-GFP-PGK-Puro

Plasmid

#110869

Purpose

Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term His8 eGFP 3C

Plasmid

#160684

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Use

Tags

His8 eGFP 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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